Recombinant Human NIPSNAP1 His Protein

SDS-Page: NIPSNAP1 Protein [NBP2-23289]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human NIPSNAP1 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-284 of Human NIPSNAP1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPRLCS ISVTARRLLG GPGPRAGDVA SAAAARFYSK DNEGSWFRSL FVHKVDPRKD AHSTLLSKKE TSNLYKIQFH NVKPEYLDAY NSLTEAVLPK LHLDEDYPCS LVGNWNTWYG EQDQAVHLWR FSGGYPALMD CMNKLKNNKE YLEFRRERSQ MLLSRRNQLL LEFSFWNEPQ PRMGPNIYEL RTYKLKPGTM IEWGNNWARA IKYRQENQEA VGGFFSQIGE LYVVHHLWAY KDLQSREETR NAAWRKRGWD ENVYYTVPLV RHMESRIMIP LKISPLQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NIPSNAP1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 35.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human NIPSNAP1 His Protein

• nipsnap homolog 1 (C. elegans)
• NIPSNAP, C. elegans, homolog 1,4-nitrophenylphosphatase domain and non-neuronal SNAP25-like 1
• NipSnap1
• protein NipSnap homolog 1

Background

NIPSNAP1 is a member of the NipSnap family of proteins that may be involved in vesicular transport. A similar protein in mice inhibits the calcium channel TRPV6, and is also localized to the inner mitochondrial membrane where it may play a role in mitochondrial DNA maintenance. A pseudogene of this gene is located on the short arm of chromosome 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NIPSNAP1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NIPSNAP1