Nitrotyrosine Antibody (39B6) - BSA Free

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immuno-peroxidase (DAB) staining for nitrotyrosine (upper row) and immuno-alkaline phosphatase (ALP) staining for 8-OHdG (bottom row), hematoxylin counterstain. Representative small intestinal tumor histopathology of Category 1 (low-grade adenoma) in ApcMin/+ (left column) and ApcMin/+ Mieap-/- (second from left column) mice, Category 2 (high-grade adenoma, second from right column) and Category 3 (adenocarcinoma, right column) in the ApcMin/+ Mieap-/- mice. Scale bars: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12472), licensed under a CC-BY license.

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.

• Reactivity: Rt, All-NA
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MA
• Clone: 39B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nitrotyrosine Antibody (39B6) - BSA Free Summary

• Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
• Specificity: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Microarray
  • Western Blot 1:1400

Reactivity Notes

Species Independent. Rat reactivity reported in scientific literature (PMID:32980492)

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Nitrotyrosine Antibody (39B6) - BSA Free

• Nitrotyrosine

Background

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nitrotyrosine Antibody (NB110-96877)(12)

Publications using NB110-96877

• Villalobos-Labra R, Liu R, Spaans F et al. Placenta-Derived Extracellular Vesicles from Preeclamptic and Healthy Pregnancies Impair ex vivo Vascular Endothelial Function Bioscience reports 2022-11-21 [PMID: 36408626]
• Przepiorski A, Vanichapol T, Espiritu E Et al. Modeling oxidative injury response in human kidney organoids Stem Cell Res Ther 2022-02-22 [PMID: 35189973]
• Wang J, Zhang W, Li M, Li X The new coumarin compound Bis 3 ameliorates cognitive disorder and suppresses brain-intestine-liver systematic oxidative stress in high-fat diet mice Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021-01-20 [PMID: 33485120]
• Ding R, Ou W, Chen C et al. Endoplasmic reticulum stress and oxidative stress contribute to neuronal pyroptosis caused by cerebral venous sinus thrombosis in rats: involvement of TXNIP/peroxynitrite-NLRP3 inflammasome activation Neurochem. Int. 2020-09-24 [PMID: 32980492] (WB, Rat)
• Yu S, Zhai J, Yu J et al. Downregulation of BACH1 Protects AGAINST Cerebral Ischemia/Reperfusion Injury through the Functions of HO-1 and NQO1 Neuroscience 2020-04-18 [PMID: 32311410] (ICC/IF)
• Wang M, Law ME, Davis BJ, et al. A novel biscoumarin compound ameliorates cerebral ischemia reperfusion-induced mitochondrial oxidative injury via Nrf2/Keap-1/ARE signaling Neuropharmacology 2019-12-21 [PMID: 31874170] (ICC/IF, IF/IHC)
• Ganguly E, Aljunaidy M, Kirschenman R Sex-Specific Effects of Nanoparticle-Encapsulated MitoQ (nMitoQ) Delivery to the Placenta in a Rat Model of Fetal Hypoxia Front. Physiol. May 24 2019 [PMID: 31178743] (ICC/IF, Rat)
• Guha S, Chaurasia S, Ramachandran C, Roy, S. SLC4A11 depletion impairs NRF2 mediated antioxidant signaling and increases reactive oxygen species in human corneal endothelial cells during oxidative stress. Sci Rep. 2017-06-22 [PMID: 28642546] (ICC/IF, Human)
• Przepiorski A, Vanichapol T, Espiritu E Et al. Modeling oxidative injury response in human kidney organoids Research Square 2021-08-24 [PMID: 27194714] (IF/IHC, Human)
• Tsuneki M, Nakamura Y, Kinjo T et al. Mieap suppresses murine intestinal tumor via its mitochondrial quality control Sci Rep 2015-07-28 [PMID: 26216032] (IHC-P)
• Gao L, Qi Hb, Kc K et al. Excessive autophagy induces the failure of trophoblast invasion and vasculature: possible relevance to the pathogenesis of preeclampsia. J. Hypertens. 2014-10-15 [PMID: 25318653]
• Gao L, Qi Hb, Kc K et al. Excessive autophagy induces the failure of trophoblast invasion and vasculature: possible relevance to the pathogenesis of preeclampsia. J. Hypertens. 2014 Oct 15 [PMID: 25318653] (ICC/IF, Human)