Nitrotyrosine Antibody (39B6) [Alexa Fluor® 647]

• Reactivity: Rt, All-NA
• Applications: ELISA, ICC/IF
• Clone: 39B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Nitrotyrosine Antibody (39B6) [Alexa Fluor® 647] Summary

• Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
• Specificity: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Species Independent. Rat reactivity reported in scientific literature (PMID:32980492)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Nitrotyrosine Antibody (39B6) [Alexa Fluor® 647]

• Nitrotyrosine

Background

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.