nNOS Antibody

Western Blot: nNOS Antibody [NB120-3511] - Analysis of 40 ug of Mouse (Lane 1) and Rat Brain (Lane 2) tissue lysate.

Immunohistochemistry-Paraffin: nNOS Antibody [NB120-3511] - Normal biopsies of deparaffinized mouse skeletal muscle tissue at dilution 1:400.

Immunohistochemistry: nNOS Antibody [NB120-3511] - Staining of bNOS in rat brain.

Immunohistochemistry-Paraffin: nNOS Antibody [NB120-3511] - Normal biopsies of deparaffinized Rat cerebellum tissue.

Product Discontinued

nNOS Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.
• Localization: Cell Membrane; Sarcolemma; sarcolemmal membrane; peripheral membrane protein. In skeletal muscle, it is localized beneath the sarcolemma of fast twitch muscle fiber by associating with the dystrophin glycoprotein complex.
• Specificity: Detects bNOS from bovine, mouse, rabbit and rat tissues. This does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:8000
  • Immunohistochemistry-Paraffin 1:200 - 400
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 155 kDa protein representing bNOS from rat brain extract. IHC: Staining of bNOS in rat brain results in staining of the anterior pituitary.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for nNOS Antibody

• bNOS
• brain
• Constitutive NOS
• EC 1.14.13.39
• IHPS1
• Neuronal NOS
• nitric oxide synthase 1 (neuronal)
• nNOS
• N-NOS
• nNOSNOS type I
• NOS
• NOS1
• Peptidyl-cysteine S-nitrosylase NOS1

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for nNOS Antibody (NB120-3511)(3)

Publications using NB120-3511

• Gutziet O, Iluz R, Ben Asher H et al. Maternal N-Acetyl-Cysteine Prevents Neonatal Hypoxia-Induced Brain Injury in a Rat Model International journal of molecular sciences 2021-12-20 [PMID: 34948425] (WB, Rat)
• Brehmer A Classification of human enteric neurons Histochemistry and cell biology 2021-06-25 [PMID: 34170401]
• Wang T, Gao W et al. Interaction between interleukin 6 and angiotensin II receptor 1 in the hypothalamic paraventricular nucleus contributes to progression of heart failure. Mol Med Rep 2017-01-06 [PMID: 28440487] (WB, Rat)

Bioinformatics

• Gene Symbol: NOS1
• Entrez:
  • 4842(Human)
  • 18125(Mouse)
  • 24598(Rat)