non-muscle heavy chain 10 Myosin Antibody

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-06309] - Anti-MYH10 rabbit polyclonal antibody at 1:500 dilutionLane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 229 kDa

Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP3-06309] - Staining of human MYH10 in human placenta with rabbit polyclonal antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP3-06309] - Staining of human MYH10 in human kidney with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: non-muscle heavy chain 10 Myosin Antibody [NBP3-06309] - MYH10 was immunoprecipitated using:Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-MYH10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-MYH10 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 229 kDaObserved band size: 229 kDa

Product Discontinued

non-muscle heavy chain 10 Myosin Antibody Summary

• Immunogen: Produced in rabbits immunized with E. coli-derived Human non-muscle heavy chain 10 Myosin fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH10
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5 ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.0
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for non-muscle heavy chain 10 Myosin Antibody

• cellular myosin heavy chain, type B type B
• Cellular myosin heavy chain, type B
• heavy polypeptide 10, non-muscle
• MGC134913
• MGC134914
• MYH10
• Myosin heavy chain 10
• Myosin heavy chain, non-muscle IIb
• myosin heavy chain, nonmuscle type B
• myosin, heavy chain 10, non-muscle
• myosin-10
• near to the ATP binding region
• NMMHC II-b
• NMMHCB
• NMMHC-B
• NMMHC-IIB
• non-muscle heavy chain 10 Myosin
• Non-muscle myosin heavy chain B
• nonmuscle myosin heavy chain IIB
• Non-muscle myosin heavy chain IIb
• nonmuscle myosin heavy chain-B
• nonmuscle myosin II heavy chain-B

Background

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH10