Normal Goat Serum

• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Host: Goat
• Concentration: LYOPH

Normal Goat Serum Summary

• Description: Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: This product has been tested by SDS-PAGE and is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.
  
  Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 26004262)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide, 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute in 10 ml with deionized water.

Alternate Names for Normal Goat Serum

• 10% NGS
• blocking goat serum
• blocking grade
• NGS

Background

Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Normal Goat Serum (NBP2-23475)(11)

Publications using NBP2-23475

• Pyrshev K, Atamanchuk-Stavniichuk A, Kordysh M et al. Independent regulation of Piezo1 activity by principal and intercalated cells of the collecting duct The Journal of biological chemistry 2023-12-01 [PMID: 38043795]
• Gawish R, Maier B, Obermayer G et al. A neutrophil-B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4 eLife 2022-09-30 [PMID: 36178806]
• Bourget C, Adams KV, Morshead CM Reduced microglia activation following metformin administration or microglia ablation is sufficient to prevent functional deficits in a mouse model of neonatal stroke Journal of neuroinflammation 2022-06-15 [PMID: 35705953] (IHC-Fr)
• Pyrshev K, Khayyat NH, Stavniichuk A et al. ClC-K2 Cl- channel allows identification of A- and B-type of intercalated cells in split-opened collecting ducts FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-05-01 [PMID: 35349181] (IHC-Fr)
• Zhu LY, Yu LM, Zhang WH et al. Aging Induced p53/p21 in Genioglossus Muscle Stem Cells and Enhanced Upper Airway Injury Stem Cells Int 2020-03-04 [PMID: 32190060] (IHC-P, IF/IHC)
• Jiang S, Hao Z, Li X et al. Ketamine destabilizes growth of dendritic spines in developing hippocampal neurons in vitro via a Rho-dependent mechanism Mol Med Rep 2018-10-02 [PMID: 30280188] (ICC/IF)
• Amini P, Stojkov D, Felser A et al. Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production Nat Commun 2018-07-27 [PMID: 30054480]
• Li Y, He X, Li J et al. Proliferation and differentiation of direct co-culture of bone marrow mesenchymal stem cells and pigmented cells from the ciliary margin Mol Med Rep 2017-01-01 [PMID: 28440470] (ICC/IF)
• Zhuo M, Song L, Tang Y et al. Vaccination with ubiquitin-hepatitis B core antigen-cytoplasmic transduction peptide enhances the hepatitis B virus-specific cytotoxic T-lymphocyte immune response and inhibits hepatitis B virus replication in transgenic mice Mol Med Rep. 2015-05-25 [PMID: 26004262] (IHC-P)
• Morshead C Metformin Alters the Neuroinflammatory Response of Microglia Following Neonatal Stroke Thesis 2021-01-01
• Gawish R, Starkl P, Pimenov L et al. ACE2 is the critical in vivo receptor for SARS-CoV-2 in a novel COVID-19 mouse model with TNF- and IFN gamma-driven immunopathology eLife 2022-01-13 [PMID: 35023830] (IHC-P)