Npas4 Antibody

Western Blot: Npas4 Antibody [NBP1-68863] - Staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Npas4 Antibody [NBP1-68863] - NBP1-68863 (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Npas4 Antibody Summary

• Immunogen: Peptide with sequence CRFNTSKSLRRQS corresponding to internal region according to NP_849195.2.
• Epitope: CRFNTSKSLRRQS
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPAS4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human brain (frontal cortex) lysates and in mouse and rat brain lysates (calculated MW of 87.1 kDa band according to NP_849195.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Npas4 Antibody

• BHLHE79
• bHLHe79neuronal PAS domain-containing protein 4
• Class E basic helix-loop-helix protein 79
• Le-PAS
• neuronal PAS domain protein 4
• Neuronal PAS4
• NXFHLH-PAS transcription factor NXF
• PASD10PAS domain-containing protein 10

Background

NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Npas4 Antibody (NBP1-68863)(2)

Publications using NBP1-68863

• Jian D, Qin L, Gan H et al. NPAS4 Exacerbates Pyroptosis via Transcriptionally Regulating NLRP6 in the Acute Phase of Intracerebral Hemorrhage in Mice International journal of molecular sciences 2023-05-05 [PMID: 37176030] (Immunohistochemistry, Western Blot, Chromatin Immunoprecipitation (ChIP), Mouse)
• Ooe N, Saito K, Mikami N et al. Identification of a novel basic helix-loop-helix-PAS factor, NXF, reveals a Sim2 competitive, positive regulatory role in dendritic-cytoskeleton modulator drebrin gene expression. Mol Cell Biol 2004-01-01 [PMID: 14701734]

Bioinformatics

• Gene Symbol: NPAS4
• Entrez:
  • 266743(Human)
  • 225872(Mouse)
  • 266734(Rat)
• Uniprot:
  • NP_849195.2(Human)