NPM1 Antibody (FC82291)

Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse

Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK

• Reactivity: Hu, Mu, Rt, Bv, Ca, Do, Ha, Pm
• Applications: WB, ELISA, ICC/IF, WB
• Clone: FC82291
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NPM1 Antibody (FC82291) Summary

• Immunogen: rat B23 (nucleophosmin, NPM, numatrin). UniProt accession no. P06748.
• Epitope: The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of NPM1.
• Localization: Nuclear
• Specificity: Detects both the phosphorylated and the unphosphorylated B23 molecule.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoblotting 0.5ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 2-4 ug/ml
• Application Notes: WB: Detects a band of approximately 37 kDa. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  NIH 3T3 Whole Cell Lysate
  NPM1 Overexpression Lysate

Reactivity Notes

kangaroo rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for NPM1 Antibody (FC82291)

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E. This phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor alpha (RAR alpha). As a consequence, a fusion protein (60-70 kDa) is produced containing parts of the two genes. Monoclonal antibody reacting specifically with B23 is a useful tool for the study of the involvement of B23 in nuclear functions, cytoplasmic/nuclear trafficking, and centrosome duplication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPM1 Antibody (NB600-1030)(5)

Publications using NB600-1030

• Schueder F, Rivera-Molina F, Su M et al. Unraveling cellular complexity with unlimited multiplexed super-resolution imaging bioRxiv 2023-05-18
• Sun YM, Zhu SX, Chen XT, Pan Q et Al. lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing Mol Cell 2024-11-23 [PMID: 39579766]
• Wang XQD, Fan D, Han Q et al. Mutant NPM1 Hijacks Transcriptional Hubs to Maintain Pathogenic Gene Programs in Acute Myeloid Leukemia Cancer discovery 2023-03-01 [PMID: 36455589] (ICC/IF)
  
  Details:
  Cut & Run
• Luo H, Zhu G, Eshelman MA et al. HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia Molecular cell 2022-02-17 [PMID: 35180428]
• IbANez-Cabellos JS, Seco-Cervera M, Picher-Latorre C et al. Acute depletion of telomerase components DKC1 and NOP10 induces oxidative stress and disrupts ribosomal biogenesis via NPM1 and activation of the P53 pathway Biochimica et biophysica acta. Molecular cell research 2020-12-01 [PMID: 32910990] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: NPM1
• Entrez:
  • 4869(Human)
  • 18148(Mouse)
  • 25498(Rat)