NTB-A/SLAMF6/CD352 Antibody (292811) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human NTB-A/SLAMF6 Monoclonal Antibody (Catalog # MAB19081, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human NTB-A/SLAMF6 Monoclonal Antibody (Catalog # MAB19081, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 292811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NTB-A/SLAMF6/CD352 Antibody (292811) [Unconjugated] Summary

• Additional Information: Clone 292811 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NTB‑A/SLAMF6
  Leu28-Lys225
  Accession # Q96DU3
• Specificity: Detects human NTB‑A/SLAMF6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) BLAME or rhCRACC is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLAMF6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NTB-A/SLAMF6/CD352 Antibody (292811) [Unconjugated]

• Activating NK receptor
• CD352 antigen
• CD352
• KALIFLJ50657
• Ly108
• NK-T-B-antigen
• NTBA receptor
• NTBA
• NTB-A
• NTB-AMGC104953
• NTBAT- and B-cell antigen
• SF2000
• SLAM family member 6
• SLAMF6

Background

NTB-A (NK-T-B-antigen), also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature human NTB-A contains a 205 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set and one Ig-like C2-set domain. It also contains a 21 aa transmembrane segment and an 84 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). An alternately spliced isoform is truncated in the cytoplasmic domain and lacks the two ITSMs. Within the ECD, human NTB-A shares 48% aa sequence identity with mouse and rat NTB-A. The ECD of human NTB-A shares 19%‑34% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (2, 4, 5). It interacts homophilically through weak associations between the Ig-V domains (2, 5‑7). NTB-A functions as an activating coreceptor on NK and T cells (2, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of IFN-gamma by NK cells (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11).

1. Veillette, A. (2006) Immunol. Rev. 214:22.
2. Bottino, C. et al. (2001) J. Exp. Med. 194:235.
3. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
4. Munitz, A. et al. (2005) J. Immunol. 174:110.
5. Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
6. Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
7. Cao, E. et al. (2006) Immunity 25:559.
8. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
9. Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
10. Howie, D. et al. (2005) J. Immunol. 174:5931.
11. Kumar, K.R. et al. (2006) Science 312:1665.

Show all background references

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NTB-A/SLAMF6/CD352 Antibody (MAB19081)(6)

Publications using MAB19081

• S Joas, EH Parrish, CW Gnanadurai, E Lump, CM Stürzel, NF Parrish, GH Learn, U Sauermann, B Neumann, KM Rensing, D Fuchs, JM Billingsle, SE Bosinger, G Silvestri, C Apetrei, N Huot, T Garcia-Tel, M Müller-Tru, D Hotter, D Sauter, C Stahl-Henn, BH Hahn, F Kirchhoff Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity Nat Commun, 2018-04-10;9(1):1371. 2018-04-10 [PMID: 29636452] (Flow Cytometry, Human)
• Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells. AIDS Res Hum Retroviruses, 2016-07-20;33(2):93-100. 2016-07-20 [PMID: 27296670] (Neutralization, Human)
• Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2010-10-07;134(2):167-73. 2010-10-07 [PMID: 20933012] (Flow Cytometry, Human)
• Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P New B-cell CD molecules. Immunol. Lett., 2010-10-07;134(2):104-12. 2010-10-07 [PMID: 20933010] (Flow Cytometry, Human)
• Hislop AD, Palendira U, Leese AM Impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets. Blood, 2010-07-19;116(17):3249-57. 2010-07-19 [PMID: 20644117] (Flow Cytometry, Human)
• Banerjee P, Feuer G, Barker E Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells. J. Virol., 2007-07-03;81(18):9707-17. 2007-07-03 [PMID: 17609265] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SLAMF6
• Uniprot:
  • Q96DU3()