![Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).](http://images.novusbio.com/fullsize/Nuclear-Antigen-Antibody-235-1R-Immunocytochemistry-Immunofluorescence-NBP3-13912-img0002.jpg "Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of PFA-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IP |
| Clone | 235-1R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14102) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Nuclei of human myeloid leukemia biopsy cells |
| Localization | Nuclear |
| Marker | Human Cell Marker |
| Specificity | This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT. Immunohistology (Frozen): 1-2ug/ml for 30 minutes at RT Immunoprecipitation: 1-2 g/500ug protein lysate) Immunoprecipitation: 1-2 gram/500 ug protein lysate |
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| Theoretical MW | 70,80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP3-13912 | Applications | Species |
|---|---|---|
| Sun J, Hu L, Bok S et al. A vertebral skeletal stem cell lineage driving metastasis Nature 2023-09-01 [PMID: 37704733] |
Secondary Antibodies |
Isotype Controls |
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![Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).](http://images.novusbio.com/fullsize/Nuclear-Antigen-Antibody-235-1R-Immunocytochemistry-Immunofluorescence-NBP3-13912-img0001.jpg "Nuclear Antigen Antibody (235-1R) [NBP3-13912] - Immunofluorescence Analysis of MeOH-fixed HeLa cells with human Nuclear Antigen antibody (235-1R) followed by goat anti-rabbit IgG-CF488 (green).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
