Oct-3/4 Antibody (240408) [Phycoerythrin]

NTERA-2 cells were stained with Rat Anti-Human/Mouse Oct‑3/4 PE‑conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 240408
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Oct-3/4 Antibody (240408) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human Oct‑3/4
  Met1-Asn265 (Met262Leu)
  Accession # Q01860
• Specificity: Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: POU5F1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody (240408) [Phycoerythrin]

• 3-Oct
• Oct-3/4
• OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
• Oct4
• Otf3g
• Otf4
• Pou5f1

Background

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
3. Rosner, M.H. et al. (1990) Nature 345:686.
4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oct-3/4 Antibody (IC1759P)(14)

Publications using IC1759P

• F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A Sci Rep, 2019-05-31;9(1):8126. 2019-05-31 [PMID: 31148575] (Flow Cytometry, ICC, Western Blot, Human)
• M Dejosez, TP Zwaka Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts Stem Cell Res, 2017-12-02;26(0):44-46. 2017-12-02 [PMID: 29227831] (Flow Cytometry, Human)
• Vincent Le Coz, Chaobin Zhu, Aurore Devocelle, Aimé Vazquez, Claude Boucheix, Sandy Azzi et al. IGF-1 contributes to the expansion of melanoma-initiating cells through an epithelial-mesenchymal transition process Oncotarget 12/13/2016 [PMID: 27764776]
• A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells PLoS ONE, 2016-09-08;11(9):e0161229. 2016-09-08 [PMID: 27606941] (Flow Cytometry, Human)
• Behnam Ahmadian Baghbaderani, Adhikarla Syama, Renuka Sivapatham, Ying Pei, Odity Mukherjee, Thomas Fellner et al. Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications Stem Cell Reviews and Reports 8/1/2016 [PMID: 27283945]
• Hasmim Meriem, Bruno Stefania, Azzi Sandy et al. Isolation and characterization of renal cancer stem cells from patient-derived xenografts. Oncotarget 1/1/2016 [PMID: 26551931]
• Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M Phenotypic diversity of patient-derived melanoma populations in stem cell medium. Lab Invest, 2015-04-13;95(6):672-83. 2015-04-13 [PMID: 25867763] (Flow Cytometry, Human)
• Nie Y, Walsh P, Clarke D, Rowley J, Fellner T Scalable passaging of adherent human pluripotent stem cells. PLoS ONE, 2014-01-30;9(1):e88012. 2014-01-30 [PMID: 24498239] (Flow Cytometry, Human)
• Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med, 2014-01-08;6(2):212-25. 2014-01-08 [PMID: 24401838] (Flow Cytometry, Mouse)
• Tea Soon Park, Ludovic Zimmerlin, Elias T. Zambidis Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells Cytometry Part A 1/1/2013 [PMID: 22736485]
• Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells. Gene Ther, 2011-11-10;19(10):1035-40. 2011-11-10 [PMID: 22071967] (Flow Cytometry, Human)
• Nakahara M, Saeki K, Nakamura N Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition. Cloning Stem Cells, 2009-03-01;11(1):5-18. 2009-03-01 [PMID: 19090661] (Flow Cytometry, Human)
• Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Cancer Res., 2008-08-15;68(16):6533-40. 2008-08-15 [PMID: 18701476] (Flow Cytometry, Human, Mouse)
• Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells. Stem Cells, 2007-06-01;25(6):1393-401. 2007-06-01 [PMID: 17548530] (Flow Cytometry, Rat)

Bioinformatics

• Gene Symbol: POU5F1
• Uniprot:
  • Q01860()