Oligodendrocyte Marker O4 Antibody (O4) [Alexa Fluor® 488]

Rat cortical stem cells either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/ Chicken Oligodendrocyte Marker O4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1326G). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow
• Clone: O4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Oligodendrocyte Marker O4 Antibody (O4) [Alexa Fluor® 488] Summary

• Immunogen: Bovine brain corpus callosum white matter
• Specificity: Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oligodendrocyte Marker O4 Antibody (O4) [Alexa Fluor® 488]

• Oligodendrocyte Marker O4

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
4. Gard, A. et al. (1995) Dev. Biol. 167:596.
5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
8. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oligodendrocyte Marker O4 Antibody (FAB1326G)(4)

Publications using FAB1326G

• Bretheau F, Castellanos-Molina A, B�langer D et al. The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury Nature Communications 2022-10-02 [PMID: 36184639]
• Michael Sy, Alexander U. Brandt, Sung-Uk Lee, Barbara L. Newton, Judy Pawling, Autreen Golzar et al. N-acetylglucosamine drives myelination by triggering oligodendrocyte precursor cell differentiation Journal of Biological Chemistry 2020-12-01 [PMID: 33453988]
• Methodios Ximerakis, Scott L. Lipnick, Brendan T. Innes, Sean K. Simmons, Xian Adiconis, Danielle Dionne et al. Single-cell transcriptomic profiling of the aging mouse brain Nature Neuroscience 2019-10-01 [PMID: 31551601]
• Donna L. McPhie, Ralda Nehme, Caitlin Ravichandran, Suzann M. Babb, Sulagna Dia Ghosh, Alexandra Staskus et al. Oligodendrocyte differentiation of induced pluripotent stem cells derived from subjects with schizophrenias implicate abnormalities in development Translational Psychiatry 2018-10-23 [PMID: 30352993]