Oligodendrocyte Marker O4 Antibody (O4) [Phycoerythrin]

Rat cortical stem cells either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/ Chicken Oligodendrocyte Marker O4 PE‑conjugated Monoclonal Antibody (Catalog # FAB1326P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow
• Clone: O4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Oligodendrocyte Marker O4 Antibody (O4) [Phycoerythrin] Summary

• Immunogen: Bovine brain corpus callosum white matter
• Specificity: Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: IgM-specific Affinity-purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oligodendrocyte Marker O4 Antibody (O4) [Phycoerythrin]

• Oligodendrocyte Marker O4

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
4. Gard, A. et al. (1995) Dev. Biol. 167:596.
5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
8. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oligodendrocyte Marker O4 Antibody (FAB1326P)(14)

Publications using FAB1326P

• S Yao, MD Xu, Y Wang, ST Zhao, J Wang, GF Chen, WB Chen, J Liu, GB Huang, WJ Sun, YY Zhang, HL Hou, L Li, XD Sun Astrocytic lactate dehydrogenase A regulates neuronal excitability and depressive-like behaviors through lactate homeostasis in mice Nature Communications, 2023-02-09;14(1):729. 2023-02-09 [PMID: 36759610] (Flow Cytometry, Mouse)
• IC Clark, MA Wheeler, HG Lee, Z Li, LM Sanmarco, S Thaploo, CM Polonio, SW Shin, G Scalisi, AR Henry, JM Rone, F Giovannoni, M Charabati, CF Akl, DM Aleman, SEJ Zandee, A Prat, DC Douek, EA Boritz, FJ Quintana, AR Abate Identification of astrocyte regulators by nucleic acid cytometry Nature, 2023-01-04;0(0):. 2023-01-04 [PMID: 36599367] (Flow Cytometry, Mouse)
• EJ Thomason, E Suárez-Poz, FS Afshari, PA Rosenberg, JL Dupree, B Fuss Deletion of the Sodium-Dependent Glutamate Transporter GLT-1 in Maturing Oligodendrocytes Attenuates Myelination of Callosal Axons During a Postnatal Phase of Central Nervous System Development Oncogene, 2022-06-03;16(0):905299. 2022-06-03 [PMID: 35722615] (Flow Cytometry, Mouse)
• Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury iScience 8/20/2021 [PMID: 34381965]
• LM Sanmarco, MA Wheeler, C Gutiérrez-, CM Polonio, M Linnerbaue, FA Pinho-Ribe, Z Li, F Giovannoni, KV Batterman, G Scalisi, SEJ Zandee, ES Heck, M Alsuwailm, DL Rosene, B Becher, IM Chiu, A Prat, FJ Quintana Gut-licensed IFN&gamma+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes Nature, 2021-01-06;0(0):. 2021-01-06 [PMID: 33408417] (Flow Cytometry, Mouse)
• MA Wheeler, IC Clark, EC Tjon, Z Li, SEJ Zandee, CP Couturier, BR Watson, G Scalisi, S Alkwai, V Rothhammer, A Rotem, JA Heyman, S Thaploo, LM Sanmarco, J Ragoussis, DA Weitz, K Petrecca, JR Moffitt, B Becher, JP Antel, A Prat, FJ Quintana MAFG-driven astrocytes promote CNS inflammation Nature, 2020-02-12;0(0):. 2020-02-12 [PMID: 32051591] (Flow Cytometry, Transgenic Mouse)
• Chun-Cheih Chao, Cristina Gutiérrez-Vázquez, Veit Rothhammer, Lior Mayo, Michael A. Wheeler, Emily C. Tjon et al. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS Cell 12/1/2019 [PMID: 31813625]
• B Li, C Dasgupta, L Huang, X Meng, L Zhang MiRNA-210 induces microglial activation and regulates microglia-mediated neuroinflammation in neonatal hypoxic-ischemic encephalopathy Cell. Mol. Immunol., 2019-07-12;0(0):. 2019-07-12 [PMID: 31300734] (Flow Cytometry, Rat)
• Wheeler MA, Jaronen M, Covacu R et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation Cell 1/24/2019 [PMID: 30661753]
• G Pelizzo, V Veschi, M Mantelli, S Croce, V Di Benedet, P D'Angelo, A Maltese, L Catenacci, T Apuzzo, E Scavo, A Moretta, M Todaro, G Stassi, MA Avanzini, V Calcaterra Microenvironment in neuroblastoma: isolation and characterization of tumor-derived mesenchymal stromal cells BMC Cancer, 2018-11-27;18(1):1176. 2018-11-27 [PMID: 30482160] (Flow Cytometry, Human)
• Mi Shen, Wei Tang, Zheng Cao, Xuemin Cao, Fei Ding Isolation of rat Schwann cells based on cell sorting Molecular Medicine Reports 6/14/2017 [PMID: 28627700]
• Hal X. Nguyen, Usha Nekanti, Daniel L. Haus, Gabrielle Funes, Denisse Moreno, Noriko Kamei et al. Induction of early neural precursors and derivation of tripotent neural stem cells from human pluripotent stem cells under xeno-free conditions Journal of Comparative Neurology 8/15/2014 [PMID: 24715528]
• Bin JM, Leong SY, Bull SJ Oligodendrocyte precursor cell transplantation into organotypic cerebellar shiverer slices: a model to study myelination and myelin maintenance. PLoS ONE, 2012-07-20;7(7):e41237. 2012-07-20 [PMID: 22911763] (Flow Cytometry, Human)
• Yaddanapudi K, De Miranda J, Hornig M Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway. PLoS ONE, 2011-10-25;6(10):e26766. 2011-10-25 [PMID: 22046349] (Flow Cytometry, Mouse)