Oligodendrocyte Marker O4 Antibody (O4) [Unconjugated]

Images

 
Rat cortical stem cells (Catalog # NSC001) either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody ...read more
Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 µg/mL Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326) for 3 ...read more
Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Mouse ...read more
Oligodendrocyte Marker O4 was detected in immersion fixed 7 day differentiated rat cortical stem cells using 1 µg/mL Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
O4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Oligodendrocyte Marker O4 Antibody (O4) [Unconjugated] Summary

Immunogen
Bovine brain corpus callosum white matter
Specificity
Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
Source
N/A
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity Statement
IgM-specific Affinity-purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1-10 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB1326 in the following applications:

Publications
Read Publications using
MAB1326 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oligodendrocyte Marker O4 Antibody (O4) [Unconjugated]

  • Oligodendrocyte Marker O4

Background

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oligodendrocyte Marker O4 Antibody (MAB1326)(138)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Rabbit, Transgenic Mouse.

We have publications tested in 12 applications: Cell Culture, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunopanning, Neutralization, Western Blot.


Filter By Application
Cell Culture
(1)
Flow Cytometry
(6)
ICC
(62)
IF
(1)
IHC
(16)
IHC-Fr
(4)
IHC-P
(2)
Immunocytochemistry
(8)
Immunohistochemistry
(1)
Immunopanning
(1)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(32)
Mouse
(45)
Rat
(15)
Chicken
(1)
Rabbit
(1)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 138. Show All 138 Publications.
Publications using MAB1326 Applications Species
MacArthur, IC;Ma, L;Huang, CY;Bhavsar, H;Suzuki, M;Dawlaty, MM; Developmental DNA demethylation is a determinant of neural stem cell identity and gliogenic competence Science advances 2024-08-30 [PMID: 39196941] (Immunocytochemistry, Transgenic Mouse) Immunocytochemistry Transgenic Mouse
Martens, N;Zhan, N;Yam, S;Leijten, F;Palumbo, M;Caspers, M;Tiane, A;Friedrichs, S;Li, Y;Zee, L;Voortman, G;Zimetti, F;Jaarsma, D;Verschuren, L;Jonker, J;Kuipers, F;Lütjohann, D;Vanmierlo, T;Mulder, M; Supplementation of Seaweed Extracts to the Diet Reduces Symptoms of Alzheimer’s Disease in the APPswePS1?E9 Mouse Model Nutrients 2024-05-25 [PMID: 38892548] (Immunocytochemistry, Mouse) Immunocytochemistry Mouse
Salas-Lucia, F;Escamilla, S;Bianco, AC;Dumitrescu, A;Refetoff, S; Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie the Allan-Herndon-Dudley syndrome JCI insight 2024-02-20 [PMID: 38376950] (Immunocytochemistry, Human) Immunocytochemistry Human
Otero, MG;Kim, J;Kushwaha, YK;Rajewski, A;Nonis, FD;Santiskulvong, C;Bannykh, SI;Oza, H;Farooqi, HMU;Babros, M;Freeman, C;Dupuis, L;Mercimek-Andrews, S;Mendoza-Londono, R;Bresee, C;Adams, DR;Tifft, CJ;Toro, C;Khanlou, N;Gahl, WA;Salamon, N;Pierson, TM; Cellular Modeling of CLN6 with IPSC-derived Neurons and Glia bioRxiv : the preprint server for biology 2024-01-30 [PMID: 38352418] (Immunocytochemistry, Human) Immunocytochemistry Human
Deepti, A;Chackochan, BK;Sadanandan, S;Menon, AS;Mohandas, K;Vengellur, A;Sivan, U;Chakrapani, PSB; An easy and cost-effective method for the isolation and culturing of Neural Stem/Progenitor cells from the Subventricular (SVZ) and Dentate Gyrus (DG) of Adult Mouse Brain Journal of neuroscience methods 2024-01-18 [PMID: 38244848] (Flow Cytometry, ICC, Mouse) Flow Cytometry, ICC Mouse
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Pantoja, IEM;Ding, L;Leite, PEC;Marques, SA;Romero, JC;Din, DAE;Zack, DJ;Chamling, X;Smirnova, L; A novel approach to increase glial cell populations in brain microphysiological systems bioRxiv : the preprint server for biology 2023-09-15 [PMID: 37745321] (ICC, Human) ICC Human
Jaime Imitola, Ethan W. Hollingsworth, Fumihiro Watanabe, Marta Olah, Wassim Elyaman, Sarah Starossom et al. Stat1 is an inducible transcriptional repressor of neural stem cells self-renewal program during neuroinflammation Frontiers in Cellular Neuroscience 8/16/2023 [PMID: 37663126]
Ana Bura, Sara Čabrijan, Ivana Bertović, Antonija Jurak Begonja The intracellular and plasma membrane pools of phosphatidylinositol-4-monophosphate control megakaryocyte maturation and proplatelet formation Research and Practice in Thrombosis and Haemostasis 5/1/2023 [PMID: 37304829]
Emily A B Gilbert, Jessica Livingston, Emilio Garcia-Flores, Tarlan Kehtari, Cindi M Morshead Metformin Improves Functional Outcomes, Activates Neural Precursor Cells, and Modulates Microglia in a Sex-Dependent Manner After Spinal Cord Injury Stem Cells Translational Medicine 6/15/2023 [PMID: 37209417]
Show All 138 Publications.

Review for Oligodendrocyte Marker O4 Antibody (MAB1326) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB1326:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Mouse 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedPrimary oligodendrocyte cultures
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Oligodendrocyte Marker O4 Antibody (MAB1326) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Oligodendrocyte Marker O4 Products

Blogs on Oligodendrocyte Marker O4

There are no specific blogs for Oligodendrocyte Marker O4, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Verified Customer
12/19/2014
Application: IF
Species: Mouse