OPA1 Antibody (1E8-1D9) - BSA Free

Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.

Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.

Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bv, ChHa
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: 1E8-1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

OPA1 Antibody (1E8-1D9) - BSA Free Summary

• Immunogen: Human OPA1 [Swiss-Prot# O60313].
• Localization: Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion intermembrane space
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OPA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:25
  • Western Blot 1:1000
• Application Notes: In Western blot, multiple protein isoforms can be seen at ~90, 80 and 65 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 93 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for OPA1 Antibody (1E8-1D9) - BSA Free

• BERHS
• EC 3.6.5.5
• FLJ12460
• KIAA0567dynamin-like 120 kDa protein, mitochondrial
• LargeG
• lilr3
• MGM1
• mitochondrial dynamin-like GTPase
• MTDPS14
• NPG
• NPGlargeG
• NTG
• NTGmitochondrial dynamin-like 120 kDa protein
• OPA1
• optic atrophy 1 (autosomal dominant)
• Optic atrophy protein 1

Background

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OPA1 Antibody (NBP1-71656)(4)

Publications using NBP1-71656

• Acosta CH, Clemons GA, Citadin CT et al. PRMT7 can prevent neurovascular uncoupling, blood-brain barrier permeability, and mitochondrial dysfunction in repetitive and mild traumatic brain injury Experimental neurology 2023-05-15 [PMID: 37196697] (Simple Western, Mouse)
• Wu, Z, Tantray, I Et al. MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure. Mol Cell 2019-08-22 [PMID: 31378462] (IF/IHC, Mouse)
• Bollu LR, Ren J, Blessing AM et al. Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells. Cell Cycle 2014-01-01 [PMID: 25483192]
• Montaigne D, Marechal X, Coisne A et al. Myocardial Contractile Dysfunction is Associated with Impaired Mitochondrial Function and Dynamics in Type 2 Diabetic but not in Obese Patients. Circulation. 2014-06-13 [PMID: 24928681] (WB, Human)

Reviews for OPA1 Antibody (NBP1-71656) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Celeste Wu Simple Western Mouse 02/06/2023

Summary

• Application: Simple Western
• Sample Tested: Brain (hippocampus) tissue
• Species: Mouse
• Lot: A-4

reviewed by: Verified Customer WB Human 07/25/2015

Summary

• Application: Western Blot
• Sample Tested: Human prostate cancer cell line (PC3)
• Species: Human

Bioinformatics

• Gene Symbol: OPA1
• Entrez:
  • 4976(Human)
  • 74143(Mouse)
  • 171116(Rat)
• Uniprot:
  • O60313()
  • P58281(Mouse)
  • Q2TA68(Rat)