Canine Osteocalcin ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Canine Osteocalcin. No significant cross-reactivity or interference between Canine Osteocalcin and analogues was observed. |
Standard Curve Range |
0.312 ng/mL - 20 ng/mL |
Sensitivity |
0.078 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Spike Recovery |
84 - 109% |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
BGLAP |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay Plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Canine Osteocalcin ELISA Kit (Colorimetric)
Background
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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