Osteopontin/OPN Antibody (1E6)

Western Blot: Osteopontin/OPN Antibody (1E6.) [H00006696-M08] - Osteopontin/OPN Antibody (1E6) [H00006696-M08] - Analysis of SPP1 expression in Hela S3 NE (Cat # L013V3).

Western Blot: Osteopontin/OPN Antibody (1E6) [H00006696-M08] - Analysis of SPP1 expression in HepG2 (Cat # L019V1).

Western Blot: Osteopontin/OPN Antibody (1E6) [H00006696-M08] - Analysis of SPP1 expression in COLO 320 HSR (Cat # L020V1).

Western Blot: Osteopontin/OPN Antibody (1E6) [H00006696-M08] - Analysis of SPP1 expression in human stomach.

Western Blot: Osteopontin/OPN Antibody (1E6) [H00006696-M08] - Analysis of SPP1 expression in NIH/3T3 (Cat # L018V1).

ELISA: Osteopontin/OPN Antibody (1E6) [H00006696-M08] - Detection limit for recombinant GST tagged SPP1 is approximately 1ng/ml as a capture antibody.

Product Discontinued

Osteopontin/OPN Antibody (1E6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SPP1 (AAH07016.1, 1 a.a. ~ 300 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQTLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELESASSEVN
• Specificity: SPP1 - secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Osteopontin/OPN Antibody (1E6)

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPP1
• Entrez:
  • 6696(Human)
• OMIM:
  • 166490(Human)
• Uniprot:
  • AAH07016(Human)