Osteopontin/OPN Antibody (223126) [Phycoerythrin]

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Osteopontin/OPN PE-conjugated Monoclonal Antibody (Catalog # IC14331P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 223126
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Osteopontin/OPN Antibody (223126) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Osteopontin/OPN
  Ile17-Asn300
  Accession # NP_000573.1
• Specificity: Detects human Osteopontin/OPN in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant mouse Osteopontin/OPN.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteopontin/OPN Antibody (223126) [Phycoerythrin]

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), previously referred to as Transformation-associated Secreted Phosphoprotein, Bone Sialoprotein I, 2ar, 2B7, Early T Lymphocyte Activation 1 Protein, Minopotin and Calcium Oxalate Crystal Growth Inhibitor Protein, is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the Integrins alpha _v beta ₁, alpha _v beta ₃, alpha _v beta ₅, and non-RGD-mediated interaction with CD44 and the Integrins alpha ₈ beta ₁ or alpha ₉ beta ₁. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by Thrombin, Enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

1. Ann. N.Y. Acad. Sci. (1995) 760, Apr. 21.
2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Osteopontin/OPN Antibody (IC14331P)(1)

Publication using IC14331P

• Robin S. Chirackal, Muthuvel Jayachandran, Xiangling Wang, Samuel Edeh, Zejfa Haskic, Majuran Perinpam et al. Urinary extracellular vesicle-associated MCP-1 and NGAL derived from specific nephron segments differ between calcium oxalate stone formers and controls American Journal of Physiology-Renal Physiology 2019-12-01 [PMID: 31461349] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SPP1
• Uniprot:
  • NP_000573.1()