Mouse Osteopontin/OPN ELISA Kit (Colorimetric)

ELISA: Mouse Osteopontin/OPN ELISA Kit (Colorimetric) [KA0547] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Discontinued

Mouse Osteopontin/OPN ELISA Kit (Colorimetric) Summary

• Description: Spp1 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Spp1.
• Standard Curve Range: 156 to 10000 pg/mL
• Sensitivity: <10 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SPP1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse Osteopontin/OPN ELISA Kit (Colorimetric)

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Osteopontin/OPN ELISA Kit (KA0547)(3)

Publications using KA0547

• Beck GR Jr, Zerler B, Moran E. Phosphate is a specific signal for induction of osteopontin gene expression. Proc Natl Acad Sci U S A. 2000-07-18 [PMID: 10890885]
• Fisher LW, McBride OW, Termine JD, Young MF. Human bone sialoprotein. Deduced protein sequence and chromosomal localization. J Biol Chem. 1990-02-05 [PMID: 2404984]
• Kohri K, Suzuki Y, Yoshida K et al. Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin. Biochem Biophys Res Commun. 1992-04-30 [PMID: 1575754]

FAQs for Osteopontin/OPN ELISA Kit (KA0547). (Showing 1 - 1 of 1 FAQs).

1. We are in need of an ELISA kit for mouse osteopontin. It is important to know the epitopes of reactivity for the antibodies in the kit, as only certain domains of the molecule are relevant. Could you please provide this information for your kit BEK-2084-2P (and other relevant kits you may have)?
   • Both of our ELISA kits suitable for measuring Osteopontin in mouse, BEK-2084-2P and KA0547, use polyclonal antibodies as both the capture and detection antibodies, so the epitope detected is unknown but should detect along the entire length of the protein.

Bioinformatics

• Gene Symbol: SPP1