Recombinant Human Osteopontin/OPN His Protein

SDS-Page: Osteopontin Protein [NBC1-21056] - Osteopontin, 36.2 kDa (321aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Product Discontinued

Recombinant Human Osteopontin/OPN His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 17-314 of Human Osteopontin/OPN
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SPP1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 36.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Osteopontin/OPN His Protein

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SPP1