Otx2 Antibody (246826) [Alexa Fluor® 488]

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Otx2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1979G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

Otx2 Antibody (246826) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Otx2 (isoform b)
  Met1-Leu289
  Accession # P32243
• Specificity: Detects human Otx2 in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTX2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody (246826) [Alexa Fluor® 488]

• homeobox protein OTX2
• MCOPS5
• MGC45000
• orthodenticle homeobox 2
• orthodenticle homolog 2 (Drosophila)
• Orthodenticle homolog 2
• Otx2

Background

Otx2 (orthodenticle 2) is a 289 amino acid protein that is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95(7):3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otx2 Antibody (IC1979G)(3)

Publications using IC1979G

• I Fagerlund, A Dougalis, A Shakirzyan, M Gómez-Budi, A Pelkonen, H Konttinen, S Ohtonen, MF Fazaludeen, M Koskuvi, J Kuusisto, D Hernández, A Pebay, J Koistinaho, T Rauramaa, Š Lehtonen, P Korhonen, T Malm Microglia-like Cells Promote Neuronal Functions in Cerebral Organoids Cells, 2021-12-30;11(1):. 2021-12-30 [PMID: 35011686] (ICC, Human)
• YL Kuang, A Munoz, G Nalula, KE Santostefa, V Sanghez, G Sanchez, N Terada, AN Mattis, M Iacovino, C Iribarren, RM Krauss, MW Medina Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation Stem Cell Res, 2019-04-10;37(0):101434. 2019-04-10 [PMID: 30999275] (Flow Cytometry, Human)
• Q Liu, K Van Bortle, Y Zhang, MT Zhao, JZ Zhang, BS Geller, JJ Gruber, C Jiang, JC Wu, MP Snyder Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid Sci Rep, 2018-08-28;8(1):12960. 2018-08-28 [PMID: 30154523] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: OTX2
• Uniprot:
  • P32243()