Recombinant Mouse OX40 Ligand/TNFSF4 Protein

In vitro assay: TNFSF4 Protein [NBP2-26577] - Demonstration in vitro of the potency of Fc-OX40L in comparison to an agonist antibody OX86, with anti-CD3 alone serving as a negative control for co-stimulation.

SDS-Page: TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-20% reducing SDS-PAGE.

SDS-Page: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-15% reducing SDS-PAGE.

• Reactivity: Mu
• Applications: Func, PAGE
• Concentration: 2 mg/ml

Recombinant Mouse OX40 Ligand/TNFSF4 Protein Summary

• Description: A recombinant protein corresponding to TNFSF4.

  Amino Acid Sequence:
  EPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGKSGRQLSSSPAKDPPIQRLRGAVTRCEDGQLFISSYKNEYQTMEVQNNSVVIKCDGLYIIYLKGSFFQEVKIDLHFREDHNPISIPMLNDGRRIVFTVVASLAFKDKVYLTVNAPDTLCEHLQINDGELIVVQLTPGYCAPEGSYHSTVNQVPL Mouse Ig-Fc amino acid sequence, followed by linker (SGR) in bold and Extracellular mouse OX40L amino acid sequence is underlined

• Details of Functionality: In vitro proliferation assay, in vivo assays.This protein was measured by its ability to co-stimulate IL-2 secretion in the presence of anti-mCD3, and agonist antibody OX86.
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF4
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level < 0.1 EU/ug of the protein (< 0.01 ng/ug of the protein) as determined by the LAL test.

Applications/Dilutions

• Dilutions:
  • Functional reported in scientific literature (PMID 24633226)
  • In vitro assay reported in scientific literature (PMID 24633226)
  • SDS-Page
• Theoretical MW: 43.54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24633226)

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filter sterilized.
• Preservative: No Preservative
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for Recombinant Mouse OX40 Ligand/TNFSF4 Protein

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

OX40 (CD134) and its natural ligand OX40L (CD134L) belong to the tumor necrosis factor superfamily. OX40L is primarily expressed on antigen presenting cells including activated B-cells, dendritic cells, and macrophages. OX40L has been shown to be expressed on activated endothelial cells and CD4+ T-cells during antigen stimulation. OX-40 and OX-40L interactions are crucial for the generation and survival of memory CD4+ T-cells. These interactions may stimulate effector and memory CD4+ and CD8+ T-cells and suppress CD4+/CD25+ Tregs and may have a role in enhanced immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for OX40 Ligand/TNFSF4 Recombinant Protein (NBP2-26577)(2)

Publications using NBP2-26577

• Song Y, Margolles-Clark E, Bayer A, Buchwald P. Small-Molecule Modulators of the OX40-OX40L Costimulatory Protein-Protein Interaction. Br. J. Pharmacol. 2014-06-16 [PMID: 24930776] (Func, Mouse)
  
  Details:
  Figs 2, S3. The TNFSF4 (OX40L:Fc) protein was coated on plates and incubated with test compounds to identify OX40L small molecule binding partners.
• Mahmud SA, Manlove LS, Schmitz HM et al. Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells. Nat Immunol. [PMID: 24633226] (Func, In vitro, Mouse)
  
  Details:
  Citation using the Azide Free form of this antibody.

FAQs for OX40 Ligand/TNFSF4 Recombinant Protein (NBP2-26577). (Showing 1 - 1 of 1 FAQs).

1. Could you let me know the expression cell of this recombinant protein?
   • The actual cell line(s) that was used is considered a proprietary information, and therefore could be be revealed.

Bioinformatics

• Gene Symbol: TNFSF4