P2X7/P2RX7 Antibody (7G1D6) - BSA Free

Western Blot: P2X7/P2RX7 Antibody (7G1D6) [NBP2-61748] - Analysis using P2RX7 mAb against HEK293 (1) and P2RX7 (AA: 226-452)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody (7G1D6) [NBP2-61748] - Analysis of esophageal cancer tissues using P2RX7 mouse mAb with DAB staining.

Flow Cytometry: P2X7/P2RX7 Antibody (7G1D6) [NBP2-61748] - Analysis of Hela cells using P2RX7 mouse mAb (green) and negative control (red).

Western Blot: P2X7/P2RX7 Antibody (7G1D6) [NBP2-61748] - Analysis using P2RX7 mAb against human P2RX7 (AA: 226-452) recombinant protein. (Expected MW is 48.9 kDa)

Western Blot: P2X7/P2RX7 Antibody (7G1D6) [NBP2-61748] - Analysis using P2RX7 mouse mAb against A431 (1), U251 (2), Hela (3), U937 (4), and HepG2 (5) cell lysate.

ELISA: P2X7/P2RX7 Antibody (7G1D6) [NBP2-61748] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 7G1D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

P2X7/P2RX7 Antibody (7G1D6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human P2X7/P2RX7 (AA: 226-452) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: P2RX7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 68.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for P2X7/P2RX7 Antibody (7G1D6) - BSA Free

• ATP receptor
• MGC20089
• P2RX7
• P2X purinoceptor 7
• P2X7
• P2X7P2X7 receptor
• P2X7R
• P2Z receptor
• purinergic receptor P2X, ligand-gated ion channel, 7
• purinergic receptor P2X7 variant A
• Purinergic receptor

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2X7/P2RX7 Antibody (NBP2-61748)(1)

Publication using NBP2-61748

• Zhao Y, Chen X, Lyu S et al. Identification of novel P2X7R antagonists by using structure based virtual screening and cell based assays Chemical biology & drug design 2021-05-15 [PMID: 33993620]

Bioinformatics

• Gene Symbol: P2RX7