p53 Antibody (BP53-12 + DO-7)

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) [NBP2-34309] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12 + DO-7) [NBP2-34309] - Formalin-fixed, paraffin-embedded human cervical carcinoma stained with P53 Monoclonal antibody (BP53-12 +DO-7)

• Reactivity: Hu, Bv, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: BP53-12 + DO-7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

p53 Antibody (BP53-12 + DO-7) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34563)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human wild-type p53 protein (BP53-12) & Recombinant human wild type p53 protein expressed in E. coli (DO-7) (Uniprot: P04637)
• Epitope: aa 20-25
• Localization: Predominantly nuclear
• Specificity: Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
• Isotype: IgG2a Kappa/IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP53
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25-0.5 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for p53 Antibody (BP53-12 + DO-7)

• Antigen NY-CO-13
• BCC7
• FLJ92943
• LFS1
• LFS1TRP53
• p53 tumor suppressor
• p53
• P53cellular tumor antigen p53
• Phosphoprotein p53
• TP53
• transformation-related protein 53
• TRP53
• tumor protein p53
• Tumor suppressor p53

Background

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP53
• Uniprot:
  • P04637()
  • P04637()
  • P04637()