p53 Antibody (BP53-12)

Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).

Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.

• Reactivity: Hu, Ca, Ch, Ha, Pm, Mu(-), Rt(-)
• Applications: WB, Simple Western, IHC
• Clone: BP53-12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

p53 Antibody (BP53-12) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33074)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human wild-type p53 protein (Uniprot: P04637)
• Epitope: aa 16-25
• Localization: Predominantly nuclear
• Specificity: This monoclonal antibody reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP53
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in MDA-MB-231 lysate, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 60 kDa.
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for p53 Antibody (BP53-12)

• Antigen NY-CO-13
• BCC7
• FLJ92943
• LFS1
• LFS1TRP53
• p53 tumor suppressor
• p53
• P53cellular tumor antigen p53
• Phosphoprotein p53
• TP53
• transformation-related protein 53
• TRP53
• tumor protein p53
• Tumor suppressor p53

Background

Nuclear protein p53 plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutations in the evolutionarily conserved codons of the p53 tumor suppressor gene are common in diverse types of human cancer, which includes cancer of colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues. p53 maps to the 17p13.1 region of the human chromosome. Consistent with its role as a tumor suppressor gene in a wide variety of tissue types, p53 performs several different critical functions in regulating cellular growth, replication, and death. p53 positively regulates transcription by binding to specific DNA consensus sequences. These sequences are associated with several known genes including the human ribosomal gene cluster, muscle creatine kinase gene, WAF-1/CIPI/p21, and cyclin G. This sequence-specific transcriptional activation is associated with the induction of growth suppression. p53 also negatively regulates transcription of genes which have TATA box initiated promoters, likely by binding to protein components of the basal transcription machinery. Possibly through the general mechanism of transcriptional regulation, p53 affects cell behavior via several specific pathways. Wild-type p53 suppresses cell proliferation by blocking the transition from G1 to S-phase. This may occur through p53 mediated induction of a universal inhibitor of cyclin-dependent kinases, WAF-1 or CIPI. Increased expression of WAF 1/CIPI/p21 serves to inhibit phosphorylation of Rb protein by cdk complexes, resulting in failure to progress from G1 to S phase. This p53 antibody recognizes a 53kDa protein, which is identified as p53 suppressor gene product. The antibody reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p53 Antibody (NBP2-29453)(8)

Publications using NBP2-29453

• Liu L, Gong X, Zhang X et al. Resveratrol Alleviates Heat-Stress-Induced Impairment of the Jejunal Mucosa Through TLR4/MAPK Signaling Pathway in Black-Boned Chicken Poultry Science 2023-10-01 [PMID: 37980746] (Western Blot, Avian - Chicken)
• Bai X, Ye D, Shi Y et al. Neuroprotection of SRT2104 in Murine Ischemia/Reperfusion Injury Through the Enhancement of Sirt1-Mediated Deacetylation Investigative ophthalmology & visual science 2023-04-03 [PMID: 37099021] (WB, Mouse)
  
  Details:
  1:200 dilution
• Eskiler GG, Turna O, Ozkan AD et al. The response of the canine mammary simple carcinoma and carcinosarcoma cells to 5-aminolaevulinic acid-based photodynamic therapy: An in vitro study Journal of photochemistry and photobiology. B, Biology 2022-07-05 [PMID: 35810598] (WB, Canine)
• Park S, Uchida T, Tilson S et al. A Dual Conditional CRISPR/Cas9 System to Activate Gene Editing and Reduce Off-Target Effects in Human Stem Cells Molecular Therapy - Nucleic Acids 2022-04-01 [PMID: 35615005] (Simple Western)
• Guo J, Yan WR, Tang JK et al. Dietary phillygenin supplementation ameliorates aflatoxin B1-induced oxidative stress, inflammation, and apoptosis in chicken liver Ecotoxicology and environmental safety 2022-05-01 [PMID: 35405527] (WB, Chicken)
• Tordjman J, Majumder M, Amiri M et al. Tumor suppressor role of cytoplasmic polyadenylation element binding protein 2 (CPEB2) in human mammary epithelial cells BMC Cancer 2019-06-11 [PMID: 31185986] (WB, Human)
• Morphine promotes tumor angiogenesis and increases breast cancer progression Bimonte S, Barbieri A, Rea D Biomed Res Int [PMID: 26064880] (WB, Human)
• Samaga KKL, Rao GV, Chandrashekara Reddy G. Synthetic racemates of abyssinone I and II induces apoptosis through mitochondrial pathway in human cervix carcinoma cells. Bioorganic Chemistry. 2014-06-26 [PMID: 25019692] (WB, Human)
  
  Details:
  Fig 6: HeLa

Review for p53 Antibody (NBP2-29453) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/09/2017

Summary

• Application: Western Blot
• Sample Tested: MCF10A cells
• Species: Human

Bioinformatics

• Gene Symbol: TP53
• Uniprot:
  • P04637()
  • P04637()
  • P04637()