p70 S6 Kinase/S6K Antibody (2C2)

Western Blot: p70 S6 Kinase/S6K Antibody (2C2) [H00006198-M03] - Analysis of RPS6KB1 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K Antibody (2C2) [H00006198-M03] - Analysis of monoclonal antibody to RPS6KB1 on A-431 cell. Antibody concentration 10 ug/ml

Western Blot: p70 S6 Kinase/S6K Antibody (2C2) [H00006198-M03] - Analysis of RPS6KB1 expression in PC-12 (Cat # L012V1).

Western Blot: p70 S6 Kinase/S6K Antibody (2C2) [H00006198-M03] - Analysis of RPS6KB1 expression in Raw 264.7 (Cat # L024V1).

Western Blot: p70 S6 Kinase/S6K Antibody (2C2) [H00006198-M03] - Analysis of RPS6KB1 expression in A-431 (Cat # L015V1).

Sandwich ELISA: p70 S6 Kinase/S6K Antibody (2C2) [H00006198-M03] - Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

p70 S6 Kinase/S6K Antibody (2C2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RPS6KB1 (NP_003152, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL
• Specificity: RPS6KB1 (2C2)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KB1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p70 S6 Kinase/S6K Antibody (2C2)

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KB1
• Entrez:
  • 6198(Human)
• OMIM:
  • 608938(Human)
• Uniprot:
  • NP_003152(Human)