p70 S6 Kinase/S6K [p Ser424] Antibody

Product Discontinued

p70 S6 Kinase/S6K [p Ser424] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-SP-P-V).
• Modification: p Ser424
• Specificity: p70 S6 Kinase (specific to Phospho-Ser424) detects endogenous levels of p70 S6 Kinase only when phosphorylated at serine 424.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100~1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50~1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  p70 S6 Kinase/S6K Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS [pH 7.4] containing 150mM NaCl and 50% glycerol (without Mg2+ and Ca2+)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K [p Ser424] Antibody

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka) and S6K2 (S6Kb). The S6K1 isoform has two splicing forms, which are the products of alternative start codon usage. The first splicing form, p70S6K or p70S6K1, has a predicted molecular mass of 56 kDa and an apparent molecular mass (SDS-PAGE) of 70 kDa. The second isoform, p85S6K or p85S6K1, contains 23 extra amino acid residues at the N terminus, resulting from the alternative start codon. It has a predicted molecular mass of 59 kDa and an apparent molecular mass of 85 kDa. Both, p70 and p85 S6K1 are activated by multiple S/T phosphorylations in response to various mitogenic stimuli and nutrients. This is accompanied by significant mobility shift in the migration of both forms, when analysed by SDS-PAGE electrophoresis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KB1
• Entrez:
  • 6198(Human)
  • 72508(Mouse)
  • 83840(Rat)
• Uniprot:
  • P23443(Human)