![ELISA: Pancreatic trypsin inhibitor Antibody (21) - BSA Free [NBP2-23589] - The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using NBP2-23589 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Pancreatic-trypsin-inhibitor-Antibody-21-BSA-Free-ELISA-NBP2-23589-img0001.jpg "ELISA: Pancreatic trypsin inhibitor Antibody (21) - BSA Free [NBP2-23589] - The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using NBP2-23589 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.")
| Reactivity | BvSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 21 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Aprotinin from bovine lung |
| Epitope | The epitope of Clone 21 is different from the epitopes of Clone 18 and Clone 35 |
| Specificity | Clone 21 binds bovine aprotinin immobilized on a solid phase or free in solution. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTI |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: NBP2-23589 (as capture antibody coated at 2 ug/mL) forms an antibody pair with NBP2-23588 (as biotinylated detection antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 ug/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. PBS-based buffers are unsuitable for this assay and 0.2 M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers. |
| Storage | Store at 4C in the dark. |
| Buffer | 10mM Phosphate (pH 7.4) and 0.5M NaCl |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | PTI |
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")