PAP Pen

Product Discontinued

PAP Pen Summary

• Specificity: Mini Elite PAP Pen

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: To start, press the tip of the pen straight down until fluid starts soaking the tip and then draw the circle around the specimen.

Packaging, Storage & Formulations

• Storage: Store at room temperature.
• Preservative: No Preservative

Notes

Note: Before use, hold the pen point up and press it with fingertip to take blocked air out from the pen barrel to prevent overflow.
Properties
1. Heat resistance up to 120C
2. Ready to be used directly on the wet slides.
3. Applicable to microwave staining and in situ hybridization
4. Circle made by PAP pen is insoluble in alcohol and acetone.
5. Withstands rehydration and dehydration steps in alcohol.
6. If desired, circle can be removed by xylene after the staining procedure is complete.

Background

The PAP PEN is designed to provide a water repellant barrier when a circle is drawn around a specimen such as tissue sections or cells. The barrier of the PAP PEN retains antisera within the defined area and ensures that only the amount of antibody needed for sufficient reaction is used. The PAP PEN stops spreading, reduces waste and divides slides into discreet areas for immunostaining, histology, cytology, cell culture, virology and microbiology. The PAP PEN is effective for immunostaining, PAP method, ABC method, ASD method, enzyme and frozen section methods. The circle is insoluble in acetone and alcohol and can be removed if desired by xylene after the staining procedure. Since water-soluble mounting media does not influence the PAP PEN, there is no interruption in microscopic observation. Properties: 1. Heat resistance up to 120 degrees C. 2. Ready to be used directly on the wet slides. 3. Applicable to microwave staining and in situ hybridization. 4. Circle made by PAP PEN is insoluble in alcohol, acetone. 5. Withstand rehydration and dehydration steps in alcohol. 6. If desired, circle can be removed by xylene after the staining procedure is completed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

FAQs for PAP Pen (NB900-66732). (Showing 1 - 1 of 1 FAQ).

1. Do you know if this particular pen contains fluorescent ink?
   • There is a chance the pen could fluoresce as we have not yet tested the product in a fluorescent experiment. However, if you can keep the diameter of the ring wide enough to view the cover slip within the penned section that may prevent viewing the pen ink with the sample. Alternatively, the PAP pen circle can be removed if desired with xylene.