![Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of DNA damage.](http://images.novusbio.com/fullsize/PAR-pADPr-Antibody-10H-Immunocytochemistry-Immunofluorescence-NBP2-89039-img0002.jpg "Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of DNA damage.")
| Reactivity | Hu, Mu, Rt, DrSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 10H |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Protein A-affinity purified from supernatant. |
| Immunogen | Purified poly(ADP-ribose). |
| Specificity | Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme. |
| Isotype | IgG3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (paraffin sections): dilution buffer: 5% milk (non fat dried milk) in PBS to a final concentration of 5-20 ug/ml). Western Blot: incubate 2.5 ug/ml in PBS, 0.05% Tween20, 5% milk (non fat dried milk)). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
| Publication using NBP2-89039 | Applications | Species |
|---|---|---|
| Huang G, He Y, Hong L et al. Restoration of NAD+ homeostasis protects C2C12 myoblasts and mouse levator ani muscle from mechanical stress-induced damage Animal cells and systems 2022-08-03 [PMID: 36046029] (IF/IHC, Mouse) | IF/IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of apoptotic cells by immunocytochemistry](http://images.novusbio.com/fullsize/PAR-pADPr-Antibody-10H-Immunocytochemistry-Immunofluorescence-NBP2-89039-img0001.jpg "Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of apoptotic cells by immunocytochemistry")
