Pax7 Antibody (PAX7/497)

Western Blot: Pax7 Antibody (PAX7/497) [NBP2-32894] - Western Blot of HeLa cell lysate using Pax7 Antibody (PAX7/497).

• Reactivity: Hu, Mu, Rt, Ch, Ze
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PAX7/497
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Pax7 Antibody (PAX7/497) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34706)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)
• Localization: Nuclear
• Marker: Rhabdomyosarcoma Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Frozen
  • Western Blot 1-2 ug/ml
• Application Notes: Use in IHC-Fr reported in scientific literature PMID: (32353175).
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Pax7 Antibody (PAX7/497)

• FLJ37460
• HUP1
• paired box 7
• paired box gene 7
• paired box homeotic gene 7
• paired box protein Pax-7
• paired domain gene 7
• PAX7 transcriptional factor
• Pax7
• PAX7B
• RMS2

Background

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax7 Antibody (NBP2-32894)(5)

Publications using NBP2-32894

• Cui W, Liu CX, Wang J et al. An oleanolic acid derivative reduces denervation-induced muscle atrophy via activation of CNTF-mediated JAK2/STAT3 signaling pathway Eur. J. Pharmacol. 2019-08-14 [PMID: 31421088] (ICC/IF, WB, Mouse)
• Ribeiro AF, Souza LS, Almeida CF et al Muscle satellite cells and impaired late stage regeneration in different murine models for muscular dystrophies. Sci Rep. 2019-08-14 [PMID: 31413358] (WB, Mouse)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.
• Suparamaniam U A Stimuli-responsive Platform for Muscle Stem Cell Culture Thesis (Flow) Stimuli-responsive Platform for Muscle Stem Cell Culture. Thesis 2019-01-01 (FLOW)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.
• Guo S, Wang Y, Wang A Identity and lineage fate of proteolipid protein 1 gene (Plp1)-expressing cells in the embryonic murine spinal cord Dev. Dyn. 2020-04-30 [PMID: 32353175] (IHC-Fr, Mouse)
• Ngan C, Quigley A, O'Connell C et al. 3D Bioprinting and Differentiation of Primary Skeletal Muscle Progenitor Cells Methods Mol Biol 2020-03-25 [PMID: 32207116]
  
  Details:
  Citation using the Azide and BSA Free format of this antibody.

Bioinformatics

• Gene Symbol: PAX7
• Uniprot:
  • P23759(Human)