PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

Western Blot: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1:2000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Immunocytochemistry/Immunofluorescence: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).

Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15 ug/mL).

PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1:2000 dilution. 1. Human Nampt (Visfatin/PBEF) (His-tagged). 2. Human Nampt (Visfatin/PBEF) (FLAG(R)-tagged). 3. Mouse Nampt (Visfatin/PBEF) (His-tagged). 4. Rat Nampt (Visfatin/PBEF) (His-tagged). 5. Human Nampt (Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.

Immunoprecipitation: PBEF/Visfatin/NAMPT Antibody (OMNI379) [NBP2-80036] - Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted and visualized by western blot with anti-human Nampt (Visfatin/PBEF) pAb.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: OMNI379
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free Summary

• Immunogen: Recombinant human PBEF/Visfatin/NAMPT (visfatin/PBEF).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAMPT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:2000 - 1:10000
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:2000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um-filtered solution in PBS, pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PBEF/Visfatin/NAMPT Antibody (OMNI379) - Azide and BSA Free

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAMPT