pCL-Eco Retrovirus Packaging Vector

pCL-Eco Retrovirus Packaging Vector [NBP2-29540]

• Reactivity: Mu, Rt
• Applications: NULL
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

pCL-Eco Retrovirus Packaging Vector Summary

• Specificity: Ecotropic (MoMuLV) for mouse and rat cells only (not human).
  Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster).
  10A1 (MuLV) for most mammalian cells (including hamster).

Applications/Dilutions

• Dilutions:
  • Retroviral Production reported in scientific literature (PMID 24760698)
• Application Notes: The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml.
  For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24760698). This product can be used in human cells to produce virions to infect non-human cells for protein production.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for pCL-Eco Retrovirus Packaging Vector

• pCL-Eco Retrovirus
• pCLECO
• pCL-Eco

Background

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for pCL-Eco Retrovirus Packaging Vector (NBP2-29540)(107)

Publications using NBP2-29540

• Jessica E. Pritchard, Juliette E. Pearce, Inge A.M. Snoeren, Stijn N.R. Fuchs, Katrin Götz, Fabian Peisker, Silke Wagner, Adam Benabid, Niklas Lutterbach, Vanessa Klöker, James S. Nagai, Monica T. Hannani, Anna K. Galyga, Ellen Sistemich, Bella Banjanin, Niclas Flosdorf, Eric Bindels, Kathrin Olschok, Katharina Biaesch, Nicolas Chatain, Neha Bhagwat, Andrew Dunbar, Rita Sarkis, Olaia Naveiras, Marie-Luise Berres, Steffen Koschmieder, Ross L. Levine, Ivan G. Costa, Hélène F.E. Gleitz, Rafael Kramann, Rebekka K. Schneider Non-canonical Hedgehog signaling mediates profibrotic hematopoiesis-stroma crosstalk in myeloproliferative neoplasms Cell Reports 2023-12-20 [PMID: 38117649]
• Miyauchi, S;Arimoto, KI;Liu, M;Zhang, Y;Zhang, DE; Reprogramming of tumor-associated macrophages via NEDD4-mediated CSF1R degradation by targeting USP18 Cell reports 2023-12-13 [PMID: 38100351]
• Miyauchi S, Kim SS, Jones RN et al. Human papillomavirus E5 suppresses immunity via inhibition of the immunoproteasome and STING pathway Cell reports 2023-05-11 [PMID: 37171962]
• Kambarev S, Borghesan E, Miller CN et al. The Brucella abortus Type IV Effector BspA Inhibits MARCH6-Dependent ERAD To Promote Intracellular Growth Infection and immunity 2023-04-27 [PMID: 37129527]
• Kamimoto K, Adil MT, Jindal K et al. Gene regulatory network reconfiguration in direct lineage reprogramming Stem cell reports 2022-12-26 [PMID: 36584685] (RetVir)
• Plenker D. Functional analysis of CD74-NRG1-a new recurrent oncogenic gene fusion in lung adenocarcinoma Thesis. 2015-01-01 (RetVir)
• Wang C, Feng X, Su D et al. Integrated screens uncover a cell surface tumor suppressor gene KIRREL involved in Hippo pathway Proceedings of the National Academy of Sciences 2022-06-21 [PMID: 35704761]
• Borghesan E, Smith EP, Myeni S Et al. A Brucella effector modulates the Arf6-Rab8a GTPase cascade to promote intravacuolar replication The EMBO journal [PMID: 34423453]
• Pease NA, Nguyen PHB, Woodworth MA et al. Tunable, division-independent control of gene activation timing by a polycomb switch Cell reports 2021-03-23 [PMID: 33761349]
• Nazari M Plasmonically enhanced photonic inactivation of pathogens Nanoscale 2021-02-04 [PMID: 33538743]
• Qing Y, Dong L, Gao L, et al. R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis Molecular cell 2021-01-06 [PMID: 33434505]
• Xu N, Palmer DC, Robeson AC, et al. STING agonist promotes CAR T cell trafficking and persistence in breast cancer The Journal of experimental medicine 2021-02-01 [PMID: 33382402]
• Leimkuhler NB, Gleitz HFE, Ronghui L et al. Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis Cell stem cell 2020-12-09 [PMID: 33301706]
• Moroney JB, Vasudev A, Pertsemlidis A et al. Integrative transcriptome and chromatin landscape analysis reveals distinct epigenetic regulations in human memory B cells Nat Commun 2020-10-28 [PMID: 33116135] (RetVir)
• Bonacina F, Martini E, Svecla M et al. Adoptive transfer of CX3CR1 transduced-T regulatory cells improves homing to the atherosclerotic plaques and dampens atherosclerosis progression Cardiovasc. Res. 2020-09-15 [PMID: 32931583]
• Evgin L, Huff AL, Wongthida P et al. Oncolytic virus-derived type I interferon restricts CAR T cell therapy Nat Commun 2020-06-24 [PMID: 32581235]
• Shen C, Sheng Y, Zhu AC et al. RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia Cell Stem Cell 2020-05-10 [PMID: 32402250] (RetVir, Human)
• Gan H, Shen T, Chupp DP et al. B cell Sirt1 deacetylates histone and non-histone proteins for epigenetic modulation of AID expression and the antibody response Sci Adv 2020-04-01 [PMID: 32270032]
• Smith EP, Cotto-Rosario A, Borghesan E et al. Epistatic Interplay between Type IV Secretion Effectors Engages the Small GTPase Rab2 in the Brucella Intracellular Cycle mBio 2020-03-31 [PMID: 32234817]
• FernAndez-Messina L, RodrIguez-GalAn A, de YEbenes VG et al. Transfer of extracellular vesicle-microRNA controls germinal center reaction and antibody production EMBO Rep. 2020-02-19 [PMID: 32073750] (Mouse)
• Woroniecka KI, Rhodin KE, Dechant CA et al. 4-1BB Agonism Averts TIL Exhaustion and Licenses PD-1 Blockade in Glioblastoma and Other Intracranial Cancers Clin. Cancer Res. 2019-12-23 [PMID: 31871298] (Human)
• Guo C Single-Cell Resolution Mechanistic Analyses of Direct Lineage Reprogramming Arterioscler Thromb Vasc Biol 2019-07-26 [PMID: 31340667]
• Federman RS, Boguraev AS, Heim EN, DiMaio D Biologically Active Ultra-Simple Proteins Reveal Principles of Transmembrane Domain Interactions J. Mol. Biol. 2019-07-10 [PMID: 31301406] (Human)
  
  Details:
  6 ul was used for each transfection in 293HEKT cells.
• Zhu P, Zhang C, Gao Y et al. The transcription factor Slug represses p16Ink4a and regulates murine muscle stem cell aging Nat Commun 2019-06-12 [PMID: 31189923]
• Luong-Gardiol, N;Siddiqui, I;Pizzitola, I;Jeevan-Raj, B;Charmoy, M;Huang, Y;Irmisch, A;Curtet, S;Angelov, GS;Danilo, M;Juilland, M;Bornhauser, B;Thome, M;Hantschel, O;Chalandon, Y;Cazzaniga, G;Bourquin, JP;Huelsken, J;Held, W; Gamma-Catenin-Dependent Signals Maintain BCR-ABL1+ B Cell Acute Lymphoblastic Leukemia Cancer Cell 2019-04-15 [PMID: 30991025] (RetVir)
• Abraham TS, Flickinger JC, Waldman SA, Snook AE. TCR Retrogenic Mice as a Model To Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen GUCY2C. J. Immunol. 2019-01-14 [PMID: 30642983] (RetVir)
• Biddy BA, Kong W, Kamimoto K et al. Single-cell mapping of lineage and identity in direct reprogramming Nature 2018-12-01 [PMID: 30518857] (RetVir, Human)
• Kenneth KH Ng, Mary A Yui, Arnav Mehta, Sharmayne Siu, Blythe Irwin, Shirley Pease, Satoshi Hirose, Michael B Elowitz, Ellen V Rothenberg, Hao Yuan Kueh, Harinder Singh, Arup K Chakraborty A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment eLife 2018-11-13 [PMID: 30457103]
• Suryadevara CM, Desai R, Farber SH et al. Preventing Lck activation in CAR T cells confers Treg resistance but requires 4-1BB signaling for them to persist and treat solid tumors in non-lymphodepleted hosts. Clin. Cancer Res. 2018-11-13 [PMID: 30425092] (RetVir)
• Knolle MD, Chin SB, Rana BMJ et al. MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.. Front Immunol. 2018-10-09 [PMID: 30356668] (RetVir, Human)
• Tang J, Zhou H, Sahay k et al. Obesity-associated family with sequence similarity 13, member A (FAM13A) is dispensable for adipose development and insulin sensitivity.. Int J Obes (Lond). 2018-10-09 [PMID: 30301961] (RetVir, Human)
• Elia AR, Grioni M, Basso V et al. Targeting tumor vasculature with TNF leads effector T cells to the tumor and enhances therapeutic efficacy of immune checkpoint blockers in combination with ACT. Clin. Cancer Res. 2018-02-28 [PMID: 29490991] (RetVir)
• Xie X, Deliorman M, Qasaimeh MA, Percipalle P. The relative composition of actin isoforms regulates cell surface biophysical features and cellular behaviors. Biochim. Biophys. Acta. 2018-02-02 [PMID: 29410074] (Human)
• X Zhou, RA Franklin, M Adler, JB Jacox, W Bailis, JA Shyer, RA Flavell, A Mayo, U Alon, R Medzhitov Circuit Design Features of a Stable Two-Cell System Cell, 2018-02-01;172(4):744-757.e17. 2018-02-01 [PMID: 29398113]
• Hones JM, Thivakaran A, Botezatu L et al. Enforced GFI1 expression impedes human and murine leukemic cell growth Sci Rep. 2017-11-16 [PMID: 29147018] (RetVir)
• Nazari M, Xi M, Lerch S, Alizadeh MH et al. Plasmonic Enhancement of Selective Photonic Virus Inactivation Sci Rep 2017-09-20 [PMID: 28931903] (RetVir)
• He L, Steinocher H, Shelar A, Cohen EB et al. Single methyl groups can act as toggle switches to specify transmembrane Protein-protein interactions Elife 2017-09-04 [PMID: 28869036] (RetVir)
• Park S, Lee S, Lee CG et al. Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5. Nat Commun 2017-07-12 [PMID: 28855737] (RetVir, Mouse)
• Miller CN, Smith EP, Cundiff JA et al. A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication Cell Host Microbe 2017-08-23 [PMID: 28844886] (RetVir, Mouse)
• Karabadzhak AG, Petti LM, Barrera FN et al. Two transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF ? receptor in an active dimeric conformation Proc. Natl. Acad. Sci. U.S.A. 2017-08-29 [PMID: 28808001] (RetVir)
• Kurkewich JL, Hansen J, Klopfenstein N et al. The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis PLoS Genet. 2017-07-01 [PMID: 28704388] (RetVir, Mouse)
• Wang Y, Skibbe JR, Hu C et al. ALOX5 exhibits anti-tumor and drug-sensitizing effects in MLL-rearranged leukemia. Sci Rep 2017-05-12 [PMID: 28500307] (Human)
• Zan H, Tat C, Qiu Z et al. Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination. Nat Commun. 2017-02-08 [PMID: 28176781] (Mouse)
• Fang V, Chaluvadi VS, Ramos-Perez WD et al. Gradients of the signaling lipid S1P in lymph nodes position natural killer cells and regulate their interferon-gamma response Nat. Immunol. 2017-01-01 [PMID: 27841869] (RetVir, Human)
• Textor A, Schmidt K, Kloetzel PM et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J. Exp. Med. 2016-10-03 [PMID: 27697836]
• Kueh HY, Yui MA, Ng KK et al. Asynchronous combinatorial action of four regulatory factors activates Bcl11b for T cell commitment Nat. Immunol. 2016-08-01 [PMID: 27376470]
• Garetto S, Sardi C, Martini E et al. Tailored chemokine receptor modification improves homing of adoptive therapy T cells in a spontaneous tumor model. Oncotarget. 2016-05-10 [PMID: 27177227]
• Jiang X, Hu C, Arnovitz S et al. miR-22 has a potent anti-tumour role with therapeutic potential in acute myeloid leukaemia. Nat Commun. 2016-04-27 [PMID: 27116251]
• Kurkewich JL, Bikorimana E, Nguyen T et al. The mirn23a microRNA cluster antagonizes B cell development. J. Leukoc. Biol. 2016-04-15 [PMID: 27084569]
• Ludwig KR, Dahl R, Hummon AB. Evaluation of the mirn23a Cluster through an iTRAQ-based Quantitative Proteomic Approach. J. Proteome Res. 2016-04-06 [PMID: 27028342]
• Huang H, Jiang X, Wang J et al. Identification of MLL-fusion/MYC - miR-26 - TET1 signaling circuit in MLL-rearranged leukemia. Cancer Lett. 2016-03-28 [PMID: 26791235] (RetVir, Human)
• Park HJ, Li J, Hannah R et al. Cytokine-induced megakaryocytic differentiation is regulated by genome-wide loss of a uSTAT transcriptional program. EMBO J. 2015-12-23 [PMID: 26702099] (Mouse)
• Matveeva EA, Venkova LS, Chernoivanenko IS, Minin AA. Vimentin is involved in regulation of mitochondrial motility and membrane potential by Rac1. Biol Open 2015-09-14 [PMID: 26369929] (RetVir)
• Robert A, Rossow MJ, Hookway C et al. Vimentin filament precursors exchange subunits in an ATP-dependent manner Proc. Natl. Acad. Sci. U.S.A. 2015-07-07 [PMID: 26109569] (RetVir)
• Xie X, Stubbington MJ, Nissen JK et al. The Regulatory T Cell Lineage Factor Foxp3 Regulates Gene Expression through Several Distinct Mechanisms Mostly Independent of Direct DNA Binding PLoS Genet. 2015-05-01 [PMID: 26107960] (RetVir)
• Lei X, Callaway M, Zhou H et al. Obesity associated Lyplal1 gene is regulated in diet induced obesity but not required for adipocyte differentiation. Mol. Cell. Endocrinol. 2015-05-06 [PMID: 25958046] (RetVir, Human)
• Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care Riccione K, Suryadevara CM, Snyder D J Vis Exp [PMID: 25741761] (RetVir, Human)
• Pone EJ, Lam T, Lou Z et al. B cell rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses J. Immunol. 2015-04-01 [PMID: 25740947] (RetVir)
• Kamimoto K, Hoffmann CM, Morris S CellOracle: Dissecting cell identity via network inference and in silico gene perturbation bioRxiv Feb 17 2020 (RetVir, Mouse)
  
  Details:
  E13.5 C57BL/6J embryos
• Hookway C, Ding L, Davidson MW et al. Microtubule-dependent transport and dynamics of vimentin intermediate filaments Mol. Biol. Cell. 2015-02-25 [PMID: 25717187] (RetVir)
• van Dam TP DNA polymerase beta prevents AID-instigated mutagenic non-canonical mismatch DNA repair bioRxiv 2020-01-01 (RetVir)
• Roy L, Bikorimana E, Lapid D et al. MiR-24 Is Required for Hematopoietic Differentiation of Mouse Embryonic Stem Cells PLoS Genet. 2015-02-01 [PMID: 25634354]
  
  Details:
  pCL-Eco Retrovirus Packaging Vector (10045P) alongwith retroviral plasmids were co-transfected into 293FT cells for retroviral production of MigR1-EGFP, MigR1-Trib3, MSCV-EGFP, and MSCV-mirn23a.
• Huichalaf Claudia, Micheloni Stefano, Ferri Giulia et al. DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level. PLoS One. 2014-01-01 [PMID: 25545674]
• Panyasing Y, Goodell C, Kittawornrat A et al. Influenza A Virus Surveillance Based on Pre-Weaning Piglet Oral Fluid Samples. Transbound Emerg Dis. 2014-12-09 [PMID: 25488821]
• Godefroy E, Gallois A, Idoyaga J et al. Activation of Toll-like Receptor-2 by Endogenous Matrix Metalloproteinase-2 Modulates Dendritic-Cell-Mediated Inflammatory Responses. Cell Rep. 2014-12-11 [PMID: 25466255]
• Textor A, Listopad J, Wuhrmann LL et al. Efficacy of CAR T cell therapy in large tumors relies upon stromal targeting by IFN-gamma. Cancer Res. 2014-10-08 [PMID: 25297631] (RetVir)
• Schoenen H, Huber A, Sonda N et al. Differential Control of Mincle-Dependent Cord Factor Recognition and Macrophage Responses by the Transcription Factors C/EBPb and HIF1a. J Immunol. 2014-08-25 [PMID: 25156364] (RetVir)
  
  Details:
  pCL-Eco Retrovirus Packaging Vector used for preparing the vector containing virus particles by cotransfection of Phoenix cells with the packaging pCL-Eco and MigR1/eGFP overexpression vector with calcium phosphate method.
• Morris SA, Cahan P, Li H et al. Dissecting Engineered Cell Types and Enhancing Cell Fate Conversion via CellNet. Cell. 2014-08-14 [PMID: 25126792] (RetVir)
  
  Details:
  pCL-Eco Retrovirus Packaging Vector used for packaging of Foxa1, Hnf4a, and Hnf4a-t2a-Foxa1 retroviruses.
• Van Allen EM, Wagle N, Stojanov P et al. Whole-exome sequencing and clinical interpretation of formalin-fixed, paraffin-embedded tumor samples to guide precision cancer medicine. Nat. Med. 2014-05-18 [PMID: 24836576] (RetVir, Human)
  
  Details:
  The pCL-Eco vector was transfected into HEk 293T cells.
• Leitman J, Shenkman M, Gofman Y et al. Herp coordinates compartmentalization and recruitment of HRD1 and misfolded proteins for ERAD. Mol. Biol. Cell. 2014-01-29 [PMID: 24478453] (RetVir, Human)
  
  Details:
  The pCL-Eco vector was used to transfect HEk293T cells. pCL-Eco Retrovirus Packaging Vector (Imgenex) were used for infections.
• Gaebler C, Gruell H, Velinzon K et al. Isolation of HIV-1-reactive antibodies using cell surface-expressed gp160deltac(BaL.). J Immunol Methods 2013-11-29 [PMID: 24041474] (Human)
  
  Details:
  Transfection
• Xu N OPTIMIZING AND UNDERSTANDING CHECKPOINT INHIBITION THERAPY AND CHIMERIC ANTIGEN RECEPTOR THERAPY AGAINST BREAST CANCER Thesis 2020-01-01
• Calero-Nieto FJ, Ng FS, SWilson NK et al. Key regulators control distinct transcriptional programmes in blood progenitor and mast cells. EMBO J. 2014-04-23 [PMID: 24760698] (RetVir, Mouse)
  
  Details:
  Mouse bone marrow cells, Fig S8A.
• Wang Y, Speier JS, Engram-Pearl J, Wilson RB. Introduction of Mismatches in a Random shRNA-Encoding Library Improves Potency for Phenotypic Selection. PLoS ONE. 2014-02-05 [PMID: 24498319] (RetVir, Human)
  
  Details:
  293T cells were cotransfected with pCL-Eco and the pSiren library. Supernatants was havested to infect FL4.12 cells. DNA Cell Culture, Retroviral Transduction:..The pSiren (Clontech) library was co-transfected with an ecotropic retroviral packaging plasmi
• Byles V, Covarrubias AJ, Ben-Sahra I et la. The TSC-mTOR pathway regulates macrophage polarization. Nat Commun 2013-01-01 [PMID: 24280772] (Human)
  
  Details:
  Transfection
• Porlan E, Morante-Redolat JM, Marques-Torrejon Ma et al. Transcriptional repression of Bmp2 by p21Waf1/Cip1 links quiescence to neural stem cell maintenance. Nat Neurosci 2013-11-01 [PMID: 24097040] (Mouse)
  
  Details:
  Transfection
• Zhong Shi, Malecek Karolina, Perez-Garcia Arianne, Krogsgaard Michelle. Retroviral transduction of T-cell receptors in mouse T-cells. J Vis Exp. 2010-01-01 [PMID: 21048669]
• Pujol C, Klein KA, Romanov GA et al. Yersinia pestis can reside in autophagosomes and avoid xenophagy in murine macrophages by preventing vacuole acidification. Infect Immun. 2009-06-01 [PMID: 19289509]
  
  Details:
  Transfected with 293 T cells.
• Rampias T, Sasaki C, Weinberger P et al. E6 and e7 gene silencing and transformed phenotype of human papillomavirus 16-positive oropharyngeal cancer cells. J Natl Cancer Inst. 2009-03-18 [PMID: 19276448]
  
  Details:
  Cotransfected with 293T cells.
• Cantor J, Browne CD, Ruppert R et al. CD98hc facilitates B cell proliferation and adaptive humoral immunity. Nat Immunol. 2009-04-01 [PMID: 19270713]
  
  Details:
  Transfected with EcoPack 293 packaging cells.
• Surgucheva I, Shestopalov VI, Surguchov A. Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion cells. J Biol Chem. 2008-12-26 [PMID: 18936092]
  
  Details:
  pSUPER retro plasmids were transfected into 293T cells with packaging plasmid pCL-Eco.
• Li Z, Lu J, Sun M et al. Distinct microRNA expression profiles in acute myeloid leukemia with common translocations. Proc Natl Acad Sci U S A. 2008-10-07 [PMID: 18832181] (Human)
  
  Details:
  PCL-Eco was transfected with human 293T cells.
• de Yebenes VG, Belver L, Pisano DG et al. miR-181b negatively regulates activation-induced cytidine deaminase in B cells. J Exp Med. 2008-09-29 [PMID: 18762567] (Human)
  
  Details:
  Human NIH-293T cells.
• Truhlar SM, Mathes E, Cervantes CF et al. Pre-folding IkappaBalpha alters control of NF-kappaB signaling. J Mol Biol. 2008-06-27 [PMID: 18511071] (Mouse)
  
  Details:
  Cotransfected with immortalized 3T3 mouse embryonic fibroblasts.
• Kanzaki N, Ogita H, Komura H et al. Involvement of the nectin-afadin complex in PDGF-induced cell survival. J Cell Sci. 2008-06-15 [PMID: 18505795]
• Li MV, Chen W, Poungvarin N et al. Glucose-mediated transactivation of carbohydrate response element-binding protein requires cooperative actions from Mondo conserved regions and essential trans-acting factor 14-3-3. Mol Endocrinol. 2008-07-01 [PMID: 18436566] (Human)
  
  Details:
  pCL-eco was cotransfected with retroviral plasmids into human packaging cells BOSC23.
• Yan Y, Kozak CA. Novel postentry resistance to AKV ecotropic mouse gammaretroviruses in the African pygmy mouse, Mus minutoides. J Virol. 2008-07-01 [PMID: 18417580] (Human)
  
  Details:
  1. pCL-MFG-LacZ (10048p) 2. pCL-Eco (10045p) pCL-MFG-LacZ was cotransfected with human 293 cells.
• Negre O, Fusil F, Henri A et al. Activation and inhibition of the erythropoietin receptor by a membrane-anchored erythropoietin. Exp Hematol. 2008-04-01 [PMID: 18295963]
• Yan Y, Jung YT, Wu T et al. Role of receptor polymorphism and glycosylation in syncytium induction and host range variation of ecotropic mouse gammaretroviruses. Retrovirology. 2008-01-10 [PMID: 18186934] (Human)
  
  Details:
  1. pCL-MFG-LacZ (10048p) 2. pCL-Eco (10045p) pCL-MFG-LacZ was cotransfected with human 293 cells.
• Roeb W, Boyer A, Cavenee WK et al. PAX3-FOXO1 controls expression of the p57Kip2 cell-cycle regulator through degradation of EGR1. Proc Natl Acad Sci U S A. 2007-11-13 [PMID: 17986608] (Human)
  
  Details:
  Transfected with human 293T cells.
• Jordan MS, Sadler J, Austin JE et al. Functional hierarchy of the N-terminal tyrosines of SLP-76. J Immunol. 2006-02-15 [PMID: 16456002]
• Zhang Y, Rosenberg S, Wang H et al. Conditional Fas-associated death domain protein (FADD): GFP knockout mice reveal FADD is dispensable in thymic development but essential in peripheral T cell homeostasis. J Immunol. 2005-09-01 [PMID: 16116191]
• Minaki Y, Mizuhara E, Morimoto K et al. Migrating postmitotic neural precursor cells in the ventricular zone extend apical processes and form adherens junctions near the ventricle in the developing spinal cord. Neurosci Res. 2005-07-01 [PMID: 15927725]
• Celli J, Salcedo SP, Gorvel JP. Brucella coopts the small GTPase Sar1 for intracellular replication. Proc Natl Acad Sci U S A. 2005-02-01 [PMID: 15632218]
• Jung YT, Wu T, Kozak CA. Novel host range and cytopathic variant of ecotropic Friend murine leukemia virus. J Virol. 2004-11-01 [PMID: 15507605]
• Akakura S, Singh S, Spataro M et al. The opsonin MFG-E8 is a ligand for the alphavbeta5 integrin and triggers DOCK180-dependent Rac1 activation for the phagocytosis of apoptotic cells. Exp Cell Res. 2004-01-15 [PMID: 14697347]
• Dahl R, Walsh JC, Lancki D et al. Regulation of macrophage and neutrophil cell fates by the PU.1:C/EBPalpha ratio and granulocyte colony-stimulating factor. Nat Immunol. 2003-10-01 [PMID: 12958595]
• Tepera SB, McCrea PD, Rosen JM. A beta-catenin survival signal is required for normal lobular development in the mammary gland. J Cell Sci. 2003-03-15 [PMID: 12584256]
• Geese M, Loureiro JJ, Bear JE et al. Contribution of Ena/VASP proteins to intracellular motility of listeria requires phosphorylation and proline-rich core but not F-actin binding or multimerization. Mol Biol Cell. 2002-07-01 [PMID: 12134077]
• Mullen AC, Hutchins AS, High FA et al. Hlx is induced by and genetically interacts with T-bet to promote heritable T(H)1 gene induction. Nat Immunol. 2002-07-01 [PMID: 12055627]
• Ikezoe K, Nakagawa M, Yan C et al. Apoptosis is suspended in muscle of mitochondrial encephalomyopathies. Acta Neuropathol. 2002-06-01 [PMID: 12012084]
• Welm BE, Freeman KW, Chen M et al. Inducible dimerization of FGFR1: development of a mouse model to analyze progressive transformation of the mammary gland. J Cell Biol. 2002-05-13 [PMID: 12011115]
• Nishimura M, Kakizaki M, Ono Y et al. JEAP, a novel component of tight junctions in exocrine cells. J Biol Chem. 2002-02-15 [PMID: 11733531]
• Zvara A, Fajardo JE, Escalante M et al. Activation of the focal adhesion kinase signaling pathway by structural alterations in the carboxyl-terminal region of c-Crk II. Oncogene. 2001-02-22 [PMID: 11314030]
• Naishiro Y, Yamada T, Takaoka AS et al. Restoration of epithelial cell polarity in a colorectal cancer cell line by suppression of beta-catenin/T-cell factor 4-mediated gene transactivation. Cancer Res. 2001-03-15 [PMID: 11289158]
• Kishi N, Tang Z, Maeda Y et al. Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. Int J Dev Neurosci. 2001-02-01 [PMID: 11226752]
• Kirsch KH, Georgescu MM, Shishido T et al. The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J Biol Chem. 2001-02-16 [PMID: 11067845]

FAQs for pCL-Eco Retrovirus Packaging Vector (NBP2-29540). (Showing 1 - 4 of 4 FAQs).

1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.  ..show answer..
   • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
2. I'm currently producing a retrovirus with the pCL-Eco packaging vector. However, my target cells are not getting infected, so I was thinking of switching to a lentivirus. Can I use this pCL-Eco packaging vector with a lentivirus, and if so, is it still only infectious to rodents?  ..show answer..
   • Unfortunately, we have not yet tested the pCL-Eco vector kit with lentivirus, therefore, we would not be able to provide you with any information regarding this type of vector. I am sorry for any inconvenience.
3. Can I use your packaging plasmid PCL-Eco Retrovirus packaging vector (pCLNDX Retrovirus expression Vector [NBP2-29540]) with lentiviral vector? I have been using the system of plasmids carrying the gag-pol, rev, GFP-lentivirus and vsv-g (to infect human cells). Can I use the PCL-Eco to infect mouse cells instead of vsv-g?  ..show answer..
   • Unfortunately, we have not tested lenti-virus in this packaging kit so I cannot tell you if it will work or not. There are lots of packaging kits on the market for lenti-virus so if you take a look maybe you can find one that will be certain to work. We, however, do not have a lenti-virus kit.
4. What is the packaging size limitation on Eco Retrovirus Packaging Vector NBP2-29540?  ..show answer..
   • The combined size of two inserts should not be more than 4 kb.