pCL-MFG-LacZ Retrovirus Reporter Vector

Product Discontinued

Applications/Dilutions

• Application Notes: For amplification, transform DH-5a or similar bacteria and plate on LB plates containing 50 ug/ml ampicillin. Sequence information for this plasmid is not available. This reporter vector is a part of the RetroMax expression system (NBP2-29499) and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. Please refer to the RetroMax Manual (NBP2-29399) for additional information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for pCL-MFG-LacZ Retrovirus Reporter Vector

• pCL-MFG-LacZ Reporter Vector
• pCL-MFG-LacZ Retrovirus
• pCL-MFG-LacZ

Background

The pCL-MFG-LacZ reporter vector is a part of the RetroMax expression system. It contains a full-length bacterial Beta- galactosidase (lacZ) under the control of the murine LTR (1). This plasmid can be co-transfected with any of the retroviral packaging vector, pCL-Eco, pCL-Ampho and pCL-10A1 to check the efficiency of transfection and retrovirus production. The expression of LacZ can be monitored using Beta-galactosidase assay kits. Sequence for this vector is not available.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for pCL-MFG-LacZ Retrovirus Reporter Vector (NBP2-29543)(7)

Publications using NBP2-29543

• Bartusch C, Prange R. ESCRT Requirements for Murine Leukemia Virus Release. Viruses. 2016-04-21 [PMID: 27096867]
• Choi J, Kim HM, Yoon JK et al. Identification of Porcine Endogenous Retrovirus (PERV) packaging sequence and development of PERV packaging viral vector system. J. Microbiol. 2015-05-01 [PMID: 25935307]
• Zaiss DM, Sijts AJ, Mosmann TR. Enumeration of cytotoxic CD8 T cells ex vivo during the response to Listeria monocytogenes infection. Infect Immun. 2008-10-01 [PMID: 18678661]
• Wassler MJ, Shur BD, Zhou W et al. Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells. Stem Cells. 2008-08-01 [PMID: 18511602]
• Maier S, Lutz R, Gelman L et al. Tenascin-C induction by cyclic strain requires integrin-linked kinase. Biochim Biophys Acta. 2008-06-01 [PMID: 18269918]
• Tang YL, Shen L, Qian K et al. A novel two-step procedure to expand cardiac Sca-1+ cells clonally. Biochem Biophys Res Commun. 2007-08-10 [PMID: 17577582]
• Ou W, Silver J. Role of protein disulfide isomerase and other thiol-reactive proteins in HIV-1 envelope protein-mediated fusion. Virology. 2006-07-05 [PMID: 16507315]