Recombinant Human PCNP His Protein

SDS-Page: Recombinant Human PCNP Protein [NBP1-72415] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PCNP His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-178 of Human PCNP
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PCNP
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.5), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human PCNP His Protein

• DKFZp781I24156
• PEST proteolytic signal containing nuclear protein
• PEST proteolytic signal-containing nuclear protein
• PEST-containing nuclear protein

Background

PCNP (PEST proteolytic signal containing nuclear protein) is a nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. This protein is ubiquitinated post-translationally by NIRF, an ubiquitin ligase. PCNP exsists as three isoforms produced by alternative splicing events and may be involved in cell cycle regulation. Recombinant human PCNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PCNP