PD-1 Antibody (PDCD1/1410R)

Immunohistochemistry-Paraffin: PD-1 Antibody (PDCD1/1410) [NBP2-47621] - Formalin-fixed, paraffin-embedded human Tonsil stained with PD1 (CD279) MAb (PDCD1/1410).

Product Discontinued

PD-1 Antibody (PDCD1/1410R) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human recombinant PDCD1 protein.
• Localization: Cell Surface & Cytoplasmic
• Specificity: PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDCD1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunofluorescence 1 - 2 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/mL
• Application Notes: Hu Chromosome Location: 2q37.3
  Immunohistology (Formalin-fixed) (1 - 2ug/mL for 30 minutes at RT). Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5 - 8.5, for 10-20 min followed by cooling at RT for 20 minutes.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for PD-1 Antibody (PDCD1/1410R)

• CD279 antigen
• CD279
• hPD-1
• PD1
• PD-1
• PD1hPD-l
• PDCD1
• programmed cell death 1
• programmed cell death protein 1
• Protein PD-1
• SLEB2

Background

PD1 is an inhibitory cell membrane surface receptor from the immunoglobulin superfamily. Involved in the regulation of T cell function during immunity and tolerance, PD1 inhibits T cell effector functions upon ligand binding. The protein is expressed in pro-B-cells and is thought to play a role in their differentiation as well. Studies have also suggested that this gene product may also contribute to the prevention of autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PD-1 Antibody (NBP2-47621)(1)

Publication using NBP2-47621

• Jin L, Tao H, Karachi A et al. CXCR1- or CXCR2-modified CAR T cells co-opt IL-8 for maximal antitumor efficacy in solid tumors Nat Commun 2019-09-05 [PMID: 31488817] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PDCD1