PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free

Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) [NB100-65332] - Analysis using the Biotin conjugate of NB100-65332. Staining of B7-DC transfected cells with Anti-Mouse CD273 (B7-DC) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) [NB100-65332] - Analysis using the PE conjugate of NB100-65332. Staining of non-transfected (left) and mouse B7-DC-transfected (right) cells with 0.25 ug of Rat IgG2a kappa Isotype Control PE (NBP1-43780) (open histogram) or 0.06 ug of Anti-Mouse CD273 (B7-DC) PE (filled histogram).

Product Discontinued

PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free Summary

• Immunogen: B7-DC transfected RAW264.7 cells.
• Specificity: NB100-65332 recognizes mouse CD273, a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Antibodies produced by clone TY25 are reported to block the binding of CD273 (B7-DC) to it's receptor.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PDCD1LG2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) - BSA Free

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

B7DC is a 42 kD member of the immunoglobulin receptor superfamily expressed on DC, liver and a small subset of macrophages as well as a few transformed cell lines. B7DC has been reported to be stimulatory on dendritic cells when cross linked and to inhibit T cell activation upon engaging the PD1 receptor. B7DC has also been reported to bind to an alternative receptor and to mediate T cell activation through non PD1 mediated interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD1LG2