PDGFR alpha Antibody (16A1) - BSA Free

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (16A1) [NBP1-44581] - Characterization of Ad vectors expressing human PDGFRalpha and EGFR. ARPE-19 epithelial, human foreskin fibroblasts (HFF), and human endothelial cells (HUVECs) were transduced with Ad vectors expressing tet-trans, PDGFRalpha, or EGFR as described above, incubated for 24 hr then fixed and stained with the PDGFRalpha-specific antibody AF-307-NA or EGFR-specific antibody LA-1 followed by a FITC-conjugated donkey anti-goat (Molecular Probes) or a Dylight 594-congugated goat anti-mouse secondary antibody. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002905), licensed under a CC-BY license.

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.

Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.

Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) FITC.

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) biotin / streptavidin-APC.

• Reactivity: Hu
• Applications: Flow, Func, ICC/IF, CyTOF-ready
• Clone: 16A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PDGFR alpha Antibody (16A1) - BSA Free Summary

• Additional Information: Clone 16A1 was used by HLDA to establish CD designation.
• Immunogen: CD140a-transfected NIH 3T3 cells
• Specificity: The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 2 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • In vitro assay
• Application Notes: This antibody is Cytof ready. Use in In vitro reported in scientific literature (PMID: 23028311).
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PDGFR alpha Antibody (16A1) - BSA Free

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (NBP1-44581)(1)

Publication using NBP1-44581

• Vanarsdall AL, Wisner TW, Lei H et al. PDGF Receptor-alpha Does Not Promote HCMV Entry into Epithelial and Endothelial Cells but Increased Quantities Stimulate Entry by an Abnormal Pathway PLoS Pathog 2012-09-01 [PMID: 23028311] (In vitro, Human)

Bioinformatics

• Gene Symbol: PDGFRA