PDGFR alpha Antibody (MM0004-8A89) - Azide and BSA Free

Immunohistochemistry-Paraffin: PDGF Receptor alpha Antibody (MM0004-8A89) [NB110-60969] - Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human PDGFR-a.

Product Discontinued

PDGFR alpha Antibody (MM0004-8A89) - Azide and BSA Free Summary

• Immunogen: Human recombinant PDGFR alpha (N-terminal)
• Specificity: No cross-reactivity is shown with human PDGF Receptor beta.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 0.025 mg size is provided in liquid form, PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGFR alpha Antibody (MM0004-8A89) - Azide and BSA Free

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (NB110-60969)(1)

Publication using NB110-60969

• Szubert, S;Moszynski, R;Szpurek, D;Romaniuk, B;Sajdak, S;Nowicki, M;Michalak, S; The expression of Platelet-Derived Growth Factor Receptors (PDGFRs) and their correlation with overall survival of patients with ovarian cancer Ginekol. Pol. 2019-04-12 [PMID: 30977516] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: PDGFRA
• Entrez:
  • 5156(Human)
• Uniprot:
  • P16234()