Pepsin Solution

• Applications: IHC

Pepsin Solution Summary

• Specificity: Stablized Pepsin solution for antigen retrieval

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Preparation of Working Solutions
  1. Solution Pepsin is ready to use and does not require any preparation.
  
  Protocol Recommendations
  1. Deparaffinize tissue sections and wash slides with wash buffer.
  2. Remove excess buffer from slide(s) without letting the tissue dry.
  3. Depending on the size of the tissue section add 1-2 drops of pepsin solution.
  4. Incubate slide(s) for 5-10 minutes at 37C.
  5. Wash slide(s) with wash buffer and proceed with immunostaining.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative

Background

Fixation is one of the most critical aspects of immunostaining. If the antigen is not properly fixed it will be washed out of the specimen. On the other hand, over fixation can cause severe problems such as masking or denaturation of the antigen. Formaldehyde is probably the best all-around fixative, due to its cross-linking characteristics. Length of fixation is very critical to prevent antigen masking. Immunoglobulins are especially susceptible to over fixation with formalin. In such cases, treatment with proteolytic enzyme is required to digest excess aldehyde linkages and to expose the antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

FAQs for Pepsin Solution (NB900-66724). (Showing 1 - 2 of 2 FAQ).

1. Do you have pepsin 5% solution?
   • NB900-66724 is a pepsin solution used for unmasking epitopes for IHC on paraffin-embedded tissues. I do not currently have information on its concentration.
2. Could you please let me know the concentration of NB900-66724?
   • Our pepsin solution is provided as 3.0mg/ml.