Peroxiredoxin 5 Antibody

Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Peroxiredoxin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Peroxiredoxin 5 Antibody Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 5
  Met1-Leu214
  Accession # P30044
• Specificity: Detects endogenous human and mouse Peroxiredoxin 5 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human Peroxiredoxin 1, 2, 3, or 6.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDX5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 5 Antibody

• ACR1
• ACR1Peroxisomal antioxidant enzyme
• Alu corepressor 1
• Alu co-repressor 1
• Antioxidant enzyme B166
• AOEB166
• AOEB166Peroxiredoxin V
• AOPP
• B166
• EC 1.11.1.15
• Liver tissue 2D-page spot 71B
• MGC117264
• MGC142283
• MGC142285
• Peroxiredoxin 5
• peroxiredoxin-5, mitochondrial
• PLPThioredoxin peroxidase PMP20
• PMP20
• PRDX5
• PRDX6
• PRXV
• prx-V
• SBBI10
• Thioredoxin reductase
• TPx type VI

Background

Peroxiredoxin-5 (Prx-5 or PRDX5; also AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The precursor molecule is 214 amino acids (aa) in length.  It contains an N-terminal 52 aa mitochondrial targeting (signal) sequence and a 162 aa (17 kDa) mature enzyme that shows a nuclear export signal between aa 58‑67, and a peroxisome targeting motif at aa 212‑214. There are two catalytic cysteines, one at Cys100 and a second at Cys204 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H₂O₂ and alkyl hydroperoxides. Prx5 can undergo cleavage after Ala52, generating two splice forms. One shows a deletion of aa 59‑147, while a second shows a deletion of aa 103‑146. Mature human Prx-5 is 78% aa identical to mouse Prx-5, with most differences noted over the first 52 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 5 Antibody (AF3774)(1)

Publication using AF3774

• S Stöcker, M Maurer, T Ruppert, TP Dick A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation Nat. Chem. Biol., 2017-12-18;0(0):. 2017-12-18 [PMID: 29251718]

Bioinformatics

• Gene Symbol: PRDX5
• Uniprot:
  • P30044()