Peroxiredoxin 6 Antibody (7G1)

Western Blot: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Analysis of PRDX6 on PC-3M (1) and K562 (2) using anti-PRDX6 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Staining PRDX6 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Analysis of paraffin-embedded human placenta tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.

Flow Cytometry: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Analysis of PC-3M cells with PRDX6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Staining PRDX6 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Staining PRDX6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Analysis of paraffin-embedded human liver tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Analysis of paraffin-embedded human colon cancer tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Peroxiredoxin 6 Antibody (7G1) [NBP2-75615] - Analysis of paraffin-embedded human kidney tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.

Product Discontinued

Peroxiredoxin 6 Antibody (7G1) Summary

• Immunogen: Recombinant full length protein of Human Peroxiredoxin 6. (SwissProt: P30041 Human)
• Localization: Cytoplasm. Lysosome.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDX6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for Peroxiredoxin 6 Antibody (7G1)

• 1-Cys
• 24 kDa protein
• Acidic calcium-independent phospholipase A2
• aiPLA2
• aiPLA21-Cys PRX
• Antioxidant protein 2
• AOP2
• AOP2Non-selenium glutathione peroxidase
• EC 1.11.1.15
• EC 1.11.1.7
• EC 3.1.1.-
• KIAA0106Red blood cells page spot 12
• Liver 2D page spot 40
• MGC46173
• NSGPx
• NSGPx1-Cys peroxiredoxin
• p29
• Peroxiredoxin 6
• peroxiredoxin-6
• PRDX6
• PRX

Background

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDX6