Recombinant Human Peroxiredoxin 6 His Protein

SDS-Page: Recombinant Human Peroxiredoxin 6 Protein [NBP1-30188] - Peroxiredoxin 6, 27.1 kDa (244aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: Func, PAGE
• Concentration: 1 mg/ml

Recombinant Human Peroxiredoxin 6 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-224 of Human Peroxiredoxin 6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP

• Details of Functionality: Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PRDX6
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • In vitro assay
  • SDS-Page
• Application Notes: Use In vitro reported in scientific literature (PMID 26553463).
• Theoretical MW: 27.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Analogous biological activity in mouse tissues/cells reported in scientific literature (PMID: 23792683)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Peroxiredoxin 6 His Protein

• 1-Cys
• 24 kDa protein
• Acidic calcium-independent phospholipase A2
• aiPLA2
• aiPLA21-Cys PRX
• Antioxidant protein 2
• AOP2
• AOP2Non-selenium glutathione peroxidase
• EC 1.11.1.15
• EC 1.11.1.7
• EC 3.1.1.-
• KIAA0106Red blood cells page spot 12
• Liver 2D page spot 40
• MGC46173
• NSGPx
• NSGPx1-Cys peroxiredoxin
• p29
• Peroxiredoxin 6
• peroxiredoxin-6
• PRDX6
• PRX

Background

Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Peroxiredoxin 6 Recombinant Protein (NBP1-30188)(2)

Publications using NBP1-30188

• Zimbler DL, Eddy JL, Schroeder JA, Lathem WW. Inactivation of peroxiredoxin 6 by the Pla protease of Yersinia pestis. Infect. Immun. 2015-11-09 [PMID: 26553463] (In vitro)
• Deeb RS, Nuriel T, Cheung C et al. Characterization of a Cellular Denitrase Activity that Reverses Nitration of Cyclooxygenase. Am J Physiol Heart Circ Physiol 2013-06-21 [PMID: 23792683] (WB, Mouse)

Bioinformatics

• Gene Symbol: PRDX6