Phorbol 12-myristate 13-acetate (PMA)

Product Discontinued

Applications/Dilutions

• Application Notes: PMA is soluble in acetone, DMSO, ethyl acetate, ethanol and methylene chloride, but is practically insoluble in water. The concentration of PMA in a saturated PMA-PBS solution has been reported to be 2.3 mg PMA per liter of PBS (i.e. 3.7 uM). To prepare aqueous solutions of PMA, it is best to start out with a concentrated solution of the compound in DMSO (20 mM) and dilute a very small aliquot of this solution rapidly with water or buffer. The compound may also be dissolved in a water-soluble detergent such as Cremophor EL at 37 degrees C and then diluted further with an aqueous medium. PMA solutions are sensitive to acid and alkaline conditions. PMA solutions in methylene chloride and ethyl acetate (at concentrations of 0.2 mM) or DMSO (20 mM) do not show any detectable autoxidation if stored in diffuse daylight for about 14 days at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.

Alternate Names for Phorbol 12-myristate 13-acetate (PMA)

• 12-O-Tetradecanoylphorbol-13-Acetate
• Factor A1 (Croton Oil)
• PMA
• TPA

Background

Phorbol 12-myristate 13-acetate, commonly referred to as PMA or TPA is a polyfunctional diterpene phorbol ester that has been widely used as a tumor promotor in cancer research. Other selected references include use in tissue culture as an activator of Protein Kinase C and as a differentiator of human leukemia cells and a general review of phorbol esters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.