Phospho-Tyrosine Antibody (G104) - BSA Free

Western Blot: Phospho-Tyrosine Antibody (G104) [NBP1-47562] - Western Blot analysis of Human A431 cell lysates showing detection of Phospho-Tyrosine protein using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody, Clone G104 (NBP1-47562). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (NBP1-47562) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Left: normal, right: EGF treated.

Immunohistochemistry: Phospho-Tyrosine Antibody (G104) [NBP1-47562] - Immunohistochemistry analysis using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody, Clone G104 (NBP1-47562). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (NBP1-47562) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Stratum granulosum staining in the epidermis. Some dermal staining.

Product Discontinued

Phospho-Tyrosine Antibody (G104) - BSA Free Summary

• Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
• Specificity: Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Phosphotyrosine Antibody was sufficient for detection of phosphorylated tyrosine residues in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Phospho-Tyrosine Antibody (G104) - BSA Free

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phospho-Tyrosine Antibody (NBP1-47562)(2)

Publications using NBP1-47562

• Garton AJ, Tonks NK. Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Bio Chem 274(6): 3811-3818. 1999-01-01 [PMID: 9920935]
• Garton AJ, Flint AJ, Tonks NK. Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 16(11): 6408-6418. 1996-01-01 [PMID: 8887669]