Phosphoserine Antibody

Western Blot: Phosphoserine Antibody [NB110-15157] - Analysis of calyculin A treated A431 cells,

ELISA: Phosphoserine Antibody [NB110-15157] - Signal to noise ratio of phospho-versus nonphospho-peptides in which the phosphorylation site is adjacent to phenylanine, tyrosine or trypophan.

Product Discontinued

Phosphoserine Antibody Summary

• Immunogen: Synthetic phospho-serine/threonine-phenylalanine containing peptide.
• Specificity: Recognizes phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position (Y/F/W)(S*/T*)F. Does not recognize the nonphosphorylated form of these motifs, nor does it recognize other phosphoserine or threonine-containing proteins and peptides.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM Sodium HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Concentration: 0.17 mg/ml
• Purity: Protein A purified

Alternate Names for Phosphoserine Antibody

• Phosphoserine; Phosphothreonine

Background

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine (3). RSK1, p70S6K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity (4,5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.