Plasma Cell Marker Antibody (SPM310)

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] - Human tonsil tissue. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] - Formalin-fixed paraffin-embedded human tonsil stained with plasma cell marker Monoclonal antibody (SPM310)

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) [NBP2-34359] - Formalin-paraffin human tonsil stained with Plasma Cell Marker MAb (SPM310).

• Reactivity: Hu, Rt(-)
• Applications: IHC
• Clone: SPM310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Plasma Cell Marker Antibody (SPM310) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34407)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Pancreatic cancer-related mucin
• Localization: Cytoplasmic
• Marker: Plasma Cell Marker
• Specificity: It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This monoclonal antibody superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this monoclonal antibody is not suitable for staining frozen tissues.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Note that this antibody is not suitable for staining frozen tissues.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plasma Cell Marker Antibody (NBP2-34359)(1)

Publication using NBP2-34359

• Huang R, Zeng Z, Yan P, et al. Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis Cancer cell international 2021-01-04 [PMID: 33397394] (IHC-P)

Review for Plasma Cell Marker Antibody (NBP2-34359) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Santhosh Sivajothi IHC-P Human 05/18/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Tonsil tissue
• Species: Human
• Lot: MSMIX-47PABX180411

Comments

• Comments: Heat induced antigen retrieval was performed in citrate buffer (pH 6) at 95C for 20 minutes.