Plasma Cell Marker Antibody (SPM310) - IHC-Prediluted

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) - IHC-Prediluted [NBP2-45200] - Human tonsil tissue. Heat induced antigen retrieval in citrate buffer pH 6 at 95C for 20 minutes. Image from verified customer review.

Immunohistochemistry-Paraffin: Plasma Cell Marker Antibody (SPM310) - IHC-Prediluted [NBP2-45200] - Formalin-fixed paraffin-embedded human tonsil stained with plasma cell marker Monoclonal antibody (SPM310)

• Reactivity: Hu, Rt(-)
• Applications: IHC
• Clone: SPM310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Plasma Cell Marker Antibody (SPM310) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Pancreatic cancer-related mucin
• Localization: Cytoplasmic
• Marker: Plasma Cell Marker
• Specificity: It recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4+ lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm; their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This monoclonal antibody superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this monoclonal antibody is not suitable for staining frozen tissues.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Note that this antibody is not suitable for staining frozen tissues.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.