Plasma Kallikrein/KLKB1 Native Protein

SDS-Page: Plasma Kallikrein/KLKB1 Native Protein [NBP2-12555] - From left to right: Lane 1: Kallikrein - 10 ug (reduced / heated). Lane 2: Kallikrein - 20 ug (reduced / heated). Lane 3: LMW Standard (BIO-RAD Cat. No. 161-0304). Lane 4: Kallikrein - 10 ug (reduced / no heat). Lane 5: Kallikrein - 20 ug (reduced / no heat. Lane 6: Blank. Lane 7: Kallikrein - 10 ug (non-reduced / no heat). Lane 8: Kallikrein - 20 ug (non-reduced / no heat).

Product Discontinued

Plasma Kallikrein/KLKB1 Native Protein Summary

• Description: Native Plasma Kallikrein/KLKB1
  
  Source:Human plasma
  Amino Acid Sequence:(P03952)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab285481)
• Preparation Method: Human plasma (Prepared from plasma shown to be non-reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests). MW 86 to 88 kDa.
• Details of Functionality: One unit is defined as the amount of enzyme that hydrolyzes one umol of D-Pro-Phe-Arg-pNA per minute at 25C, pH 7.8.
• Source: Human Plasma
• Protein/Peptide Type: Native Protein
• Gene: KLKB1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 15 units/mg
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl (pH 7.8), 100 mM NaCl
• Purity: >95%, by SDS-PAGE

Alternate Names for Plasma Kallikrein/KLKB1 Native Protein

• EC 3.4.21
• EC 3.4.21.34
• Fletcher factor
• kallikrein B, plasma (Fletcher factor) 1
• kininogenin
• KLK3plasma kallikrein
• KLKB1
• plasma kallikrein heavy chain
• plasma kallikrein light chain
• Plasma Kallikrein
• Plasma Prekallikrein
• PPK

Background

A serine protease that releases bradykinin from kininogen. Present in plasma as an inactive precursor prokallikrein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: KLKB1