PLGF Antibody (3G2)

Western Blot: PLGF Antibody (3G2) [NBP2-80390] - Analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3G2) [NBP2-80390] - Staining PLGF (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PLGF Antibody (3G2) [NBP2-80390] - Analysis in mouse testis tissue using PLGF antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3G2) [NBP2-80390] - Staining PLGF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PLGF Antibody (3G2) [NBP2-80390] - Analysis in rat kidney tissue using PLGF antibody. Counter stained with hematoxylin.

Product Discontinued

PLGF Antibody (3G2) Summary

• Immunogen: Recombinant protein within human PLGF aa 1-221. (SwissProt: P49763 Human; SwissProt: P49764 Mouse; SwissProt: Q63434 Rat)
• Localization: Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for PLGF Antibody (3G2)

• D12S1900
• PGF
• PGFL
• placenta growth factor
• placental growth factor
• placental growth factor, vascular endothelial growth factor-related protein
• PlGF
• PlGF-2
• PLGFplacental growth factor-like
• SHGC-10760

Background

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLGF Antibody (NBP2-80390)(1)

Publication using NBP2-80390

• Yusoff FM, Maruhashi T, Kawano KI et al. Bach1 Plays an Important Role in Angiogenesis Through Regulation of Oxidative Stress Microvascular research 2020-12-26 [PMID: 33373621] (WB, Mouse)

Bioinformatics

• Gene Symbol: PGF